Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10693 and 10694 are separated by 105 nucleotides10694 and 10695 are separated by 565 nucleotides10695 and 10696 are separated by 108 nucleotides10696 and 10697 are separated by 2620 nucleotides mRNA_2325: 10693 - Hypothetical Protein, at 613,315 to 614,433 10693 mRNA_2326: 10694 - K03439 trmB, METTL1 tRNA (guanine-N7-)-methyltransferase, at 614,539 to 615,888 10694 mRNA_2327: 10695 - Hypothetical Protein, at 616,454 to 617,684 10695 mRNA_2328: 10696 - KOG0192 Tyrosine kinase specific for activated (GTP-bound) p21cdc42Hs, at 617,793 to 619,842 10696 mRNA_2329: 10697 - K10908 POLRMT, RPO41 DNA-directed RNA polymerase, mitochondrial, at 622,463 to 626,733 10697
Group Condition mRNA_2325 mRNA_2326 mRNA_2327 mRNA_2328 mRNA_2329
10693 10694 10695 10696 10697
growth on ypd YPD -0.5 N.D. -1.4 -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -0.4 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.8 +0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.5 -0.2 N.D.
auxotrophy Dropout Complete -0.2 N.D. -0.5 +0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.4 N.D. -0.0 -0.2 N.D.
growth on oleic acid YNB Glucose -0.4 N.D. +0.1 -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. -0.5 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.7 +0.1 N.D.
auxotrophy YNB Glucose -0.0 N.D. -0.1 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.2 -0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.4 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. +0.2 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. -0.4 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.3 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.5 +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. -0.6 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. +0.3 +0.2 N.D.
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