Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10694 and 10695 are separated by 565 nucleotides10695 and 10696 are separated by 108 nucleotides10696 and 10697 are separated by 2620 nucleotides10697 and 10698 are separated by 1094 nucleotides mRNA_2326: 10694 - K03439 trmB, METTL1 tRNA (guanine-N7-)-methyltransferase, at 614,539 to 615,888 10694 mRNA_2327: 10695 - Hypothetical Protein, at 616,454 to 617,684 10695 mRNA_2328: 10696 - KOG0192 Tyrosine kinase specific for activated (GTP-bound) p21cdc42Hs, at 617,793 to 619,842 10696 mRNA_2329: 10697 - K10908 POLRMT, RPO41 DNA-directed RNA polymerase, mitochondrial, at 622,463 to 626,733 10697 mRNA_2330: 10698 - KOG0583 Serine/threonine protein kinase, at 627,828 to 630,238 10698
Group Condition mRNA_2326 mRNA_2327 mRNA_2328 mRNA_2329 mRNA_2330
10694 10695 10696 10697 10698
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.2 N.D. -3.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.7 +0.1 N.D. -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 +0.0 N.D. -0.9
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 -0.1 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.4 -0.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.2 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.0 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.6 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.5 +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 +0.0 N.D. -0.1
auxotrophy Dropout Complete N.D. -0.5 +0.0 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.2 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.8 +0.0 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.1 N.D. -0.1
growth on oleic acid YNB Glucose N.D. +0.1 -0.3 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 -0.0 N.D. +0.0
auxotrophy YNB Glucose N.D. -0.1 -0.2 N.D. +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 +0.2 N.D. +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.1 N.D. +1.0
growth on ypd YPD N.D. -1.4 -0.1 N.D. +2.4
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