Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10696 and 10697 are separated by 2620 nucleotides10697 and 10698 are separated by 1094 nucleotides10698 and 10699 are separated by 4275 nucleotides10699 and 10700 are separated by 860 nucleotides mRNA_2328: 10696 - KOG0192 Tyrosine kinase specific for activated (GTP-bound) p21cdc42Hs, at 617,793 to 619,842 10696 mRNA_2329: 10697 - K10908 POLRMT, RPO41 DNA-directed RNA polymerase, mitochondrial, at 622,463 to 626,733 10697 mRNA_2330: 10698 - KOG0583 Serine/threonine protein kinase, at 627,828 to 630,238 10698 mRNA_2331: 10699 - HMMPfam-Amidohydrolase family-PF07969,SUPERFAMILY--SSF51338,SUPERFAMILY--SSF51556, at 634,514 to 637,179 10699 mRNA_2332: 10700 - KOG1134 Uncharacterized conserved protein, at 638,040 to 641,999 10700
Group Condition mRNA_2328 mRNA_2329 mRNA_2330 mRNA_2331 mRNA_2332
10696 10697 10698 10699 10700
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -3.6 -0.5 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.9 -0.4 +0.0
growth on oleic acid YNB Glucose -0.3 N.D. +0.3 -0.5 -0.7
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.4 -0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.2 +0.0 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. +0.1 -0.6 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.3 -1.2 -0.0
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.0 -0.6 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.0 -0.4 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.2 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.9 +0.7 +0.0
auxotrophy YNB Glucose -0.2 N.D. +0.7 +0.2 -0.8
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.0 +0.6 -0.6
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.1 -0.2 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.5 -0.2 -0.4
growth on ricinoleic acid YNB Glucose -0.1 N.D. +1.0 -0.3 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.1 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.1 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.1 +0.7 -0.0
auxotrophy Dropout Complete +0.0 N.D. +0.2 +0.7 -0.1
growth on ypd YPD -0.1 N.D. +2.4 -0.3 -0.3
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