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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10705 and 10706 are separated by 970 nucleotides
10706 and 10707 are separated by 165 nucleotides
10707 and 10708 are separated by 418 nucleotides
10708 and 10709 are separated by 809 nucleotides
mRNA_2337: 10705 - BLAST general substrate transporter [Wallemia mellicola..., at 656,119 to 656,520
10705
mRNA_2338: 10706 - K05545 DUS4 tRNA-dihydrouridine synthase 4, at 657,491 to 659,083
10706
mRNA_2339: 10707 - SUPERFAMILY--SSF55298, at 659,249 to 660,620
10707
mRNA_2340: 10708 - K06927 DPH6 diphthine-ammonia ligase, at 661,039 to 662,267
10708
mRNA_2341: 10709 - K03021 RPC2, POLR3B DNA-directed RNA polymerase III subunit RPC2, at 663,077 to 667,244
10709
Group
Condition
mRNA
_2337
mRNA
_2338
mRNA
_2339
mRNA
_2340
mRNA
_2341
10705
10706
10707
10708
10709
growth on oleic acid
YNB Oleic Acid
-0.3
-0.5
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.4
-0.4
N.D.
N.D.
N.D.
growth on ypd
YPD
-0.6
-0.1
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.3
-0.4
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose
-0.1
-0.6
N.D.
N.D.
N.D.
auxotrophy
Dropout Complete
-0.1
-0.5
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.3
-0.3
N.D.
N.D.
N.D.
growth on oleic acid
YNB Glucose
-0.2
-0.4
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.7
+0.2
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.2
-0.3
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
-0.6
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.0
-0.3
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.4
+0.1
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
+0.0
-0.2
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
+0.3
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
+0.3
-0.1
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
+0.1
+0.0
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.3
+0.5
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
+0.4
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
+0.4
+0.0
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
+1.0
N.D.
N.D.
N.D.
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