Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10705 and 10706 are separated by 970 nucleotides10706 and 10707 are separated by 165 nucleotides10707 and 10708 are separated by 418 nucleotides10708 and 10709 are separated by 809 nucleotides mRNA_2337: 10705 - BLAST general substrate transporter [Wallemia mellicola..., at 656,119 to 656,520 10705 mRNA_2338: 10706 - K05545 DUS4 tRNA-dihydrouridine synthase 4, at 657,491 to 659,083 10706 mRNA_2339: 10707 - SUPERFAMILY--SSF55298, at 659,249 to 660,620 10707 mRNA_2340: 10708 - K06927 DPH6 diphthine-ammonia ligase, at 661,039 to 662,267 10708 mRNA_2341: 10709 - K03021 RPC2, POLR3B DNA-directed RNA polymerase III subunit RPC2, at 663,077 to 667,244 10709
Group Condition mRNA_2337 mRNA_2338 mRNA_2339 mRNA_2340 mRNA_2341
10705 10706 10707 10708 10709
growth on oleic acid YNB Oleic Acid -0.3 -0.5 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.4 N.D. N.D. N.D.
growth on ypd YPD -0.6 -0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.4 N.D. N.D. N.D.
auxotrophy YNB Glucose -0.1 -0.6 N.D. N.D. N.D.
auxotrophy Dropout Complete -0.1 -0.5 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.3 N.D. N.D. N.D.
growth on oleic acid YNB Glucose -0.2 -0.4 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 +0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.3 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.6 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.3 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.0 -0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.5 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.4 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +1.0 N.D. N.D. N.D.
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