Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10708 and 10709 are separated by 809 nucleotides10709 and 10710 are separated by 214 nucleotides10710 and 10711 are separated by 170 nucleotides10711 and 10712 are separated by 237 nucleotides mRNA_2340: 10708 - K06927 DPH6 diphthine-ammonia ligase, at 661,039 to 662,267 10708 mRNA_2341: 10709 - K03021 RPC2, POLR3B DNA-directed RNA polymerase III subunit RPC2, at 663,077 to 667,244 10709 mRNA_2342: 10710 - Hypothetical Protein, at 667,459 to 669,644 10710 mRNA_2343: 10711 - HMMPfam-GPR1/FUN34/yaaH family-PF01184, at 669,815 to 670,967 10711 mRNA_2344: 10712 - Hypothetical Protein, at 671,205 to 671,888 10712
Group Condition mRNA_2340 mRNA_2341 mRNA_2342 mRNA_2343 mRNA_2344
10708 10709 10710 10711 10712
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.2 -1.6 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.0 -1.4 -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 -0.6 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 -0.7 -0.3
growth on ypd YPD N.D. N.D. -0.2 -0.5 +0.4
growth on oleic acid YNB Glucose N.D. N.D. -0.1 +0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 -0.5 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.0 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.0 +0.5 -0.6
auxotrophy Dropout Complete N.D. N.D. +0.2 -0.1 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 -0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 +0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.4 -0.2 +0.1
auxotrophy YNB Glucose N.D. N.D. +0.3 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.4 +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.7 -0.1
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