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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10709 and 10710 are separated by 214 nucleotides
10710 and 10711 are separated by 170 nucleotides
10711 and 10712 are separated by 237 nucleotides
10712 and 10713 are separated by 255 nucleotides
mRNA_2341: 10709 - K03021 RPC2, POLR3B DNA-directed RNA polymerase III subunit RPC2, at 663,077 to 667,244
10709
mRNA_2342: 10710 - Hypothetical Protein, at 667,459 to 669,644
10710
mRNA_2343: 10711 - HMMPfam-GPR1/FUN34/yaaH family-PF01184, at 669,815 to 670,967
10711
mRNA_2344: 10712 - Hypothetical Protein, at 671,205 to 671,888
10712
mRNA_2345: 10713 - KOG1399 Flavin-containing monooxygenase, at 672,144 to 674,998
10713
Group
Condition
mRNA
_2341
mRNA
_2342
mRNA
_2343
mRNA
_2344
mRNA
_2345
10709
10710
10711
10712
10713
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
-0.0
-1.4
-0.3
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-0.2
-1.6
-0.1
+0.7
growth on ricinoleic acid
YNB Glucose
N.D.
-0.1
-0.6
-0.4
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
+0.1
-0.7
-0.3
+0.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
-0.0
+0.0
-0.2
-0.3
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
+0.2
+0.1
-0.1
-0.6
growth on ypd
YPD
N.D.
-0.2
-0.5
+0.4
+0.1
growth on oleic acid
YNB Glucose
N.D.
-0.1
+0.0
-0.2
+0.0
auxotrophy
Dropout Complete
N.D.
+0.2
-0.1
+0.0
-0.3
ypd buoyancy
40 hrs Median Buoyancy
N.D.
+0.0
+0.2
-0.0
-0.3
auxotrophy
YNB Glucose 75mM Methionine
N.D.
+0.4
-0.2
+0.1
-0.3
ypd buoyancy
40 hrs High Buoyancy
N.D.
-0.0
+0.4
-0.1
-0.2
growth on oleic acid
YNB Oleic Acid
N.D.
+0.1
+0.1
-0.0
-0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
-0.0
-0.0
-0.1
+0.2
auxotrophy
YNB Glucose
N.D.
+0.3
-0.1
+0.2
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
+0.0
+0.5
-0.6
+0.5
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
+0.2
-0.5
+0.1
+0.7
auxotrophy
YNB Glucose 75mM Arginine
N.D.
+0.4
+0.1
+0.1
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
+0.2
-0.0
+0.1
+0.6
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.1
+0.7
-0.1
+0.3
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
+0.3
+0.0
+0.2
+0.5
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