Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10709 and 10710 are separated by 214 nucleotides10710 and 10711 are separated by 170 nucleotides10711 and 10712 are separated by 237 nucleotides10712 and 10713 are separated by 255 nucleotides mRNA_2341: 10709 - K03021 RPC2, POLR3B DNA-directed RNA polymerase III subunit RPC2, at 663,077 to 667,244 10709 mRNA_2342: 10710 - Hypothetical Protein, at 667,459 to 669,644 10710 mRNA_2343: 10711 - HMMPfam-GPR1/FUN34/yaaH family-PF01184, at 669,815 to 670,967 10711 mRNA_2344: 10712 - Hypothetical Protein, at 671,205 to 671,888 10712 mRNA_2345: 10713 - KOG1399 Flavin-containing monooxygenase, at 672,144 to 674,998 10713
Group Condition mRNA_2341 mRNA_2342 mRNA_2343 mRNA_2344 mRNA_2345
10709 10710 10711 10712 10713
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 -1.4 -0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -1.6 -0.1 +0.7
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.6 -0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.7 -0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.0 -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 +0.1 -0.1 -0.6
growth on ypd YPD N.D. -0.2 -0.5 +0.4 +0.1
growth on oleic acid YNB Glucose N.D. -0.1 +0.0 -0.2 +0.0
auxotrophy Dropout Complete N.D. +0.2 -0.1 +0.0 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.2 -0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.4 -0.2 +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.4 -0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.1 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 -0.0 -0.1 +0.2
auxotrophy YNB Glucose N.D. +0.3 -0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 +0.5 -0.6 +0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.5 +0.1 +0.7
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 +0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.0 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.7 -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 +0.0 +0.2 +0.5
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