Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10716 and 10717 are separated by 282 nucleotides10717 and 10718 are separated by 285 nucleotides10718 and 10719 are separated by 1359 nucleotides10719 and 10720 are separated by 805 nucleotides mRNA_2348: 10716 - K00762 pyrE orotate phosphoribosyltransferase, at 682,328 to 683,011 10716 mRNA_2349: 10717 - KOG3419 Mitochondrial/chloroplast ribosomal protein S16, at 683,294 to 683,869 10717 mRNA_2350: 10718 - K14829 IPI3 pre-rRNA-processing protein IPI3, at 684,155 to 686,161 10718 mRNA_2351: 10719 - Hypothetical Protein, at 687,521 to 688,021 10719 mRNA_2352: 10720 - K02903 RP-L28e, RPL28 large subunit ribosomal protein L28e, at 688,827 to 689,561 10720
Group Condition mRNA_2348 mRNA_2349 mRNA_2350 mRNA_2351 mRNA_2352
10716 10717 10718 10719 10720
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. +0.2 -1.7
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 -0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 -1.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.3 -1.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.4 -1.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.3 -1.0
auxotrophy YNB Glucose N.D. N.D. N.D. +0.4 -0.8
auxotrophy Dropout Complete N.D. N.D. N.D. +0.4 -0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 -0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.1 +0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.0 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.1 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.2 +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.3 +0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.1 +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 +1.2
growth on ypd YPD N.D. N.D. N.D. -0.4 +1.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.0 +1.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 +1.5
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