Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10718 and 10719 are separated by 1359 nucleotides10719 and 10720 are separated by 805 nucleotides10720 and 10721 are separated by 410 nucleotides10721 and 10722 are separated by 132 nucleotides mRNA_2350: 10718 - K14829 IPI3 pre-rRNA-processing protein IPI3, at 684,155 to 686,161 10718 mRNA_2351: 10719 - Hypothetical Protein, at 687,521 to 688,021 10719 mRNA_2352: 10720 - K02903 RP-L28e, RPL28 large subunit ribosomal protein L28e, at 688,827 to 689,561 10720 mRNA_2353: 10721 - SUPERFAMILY--SSF88713, at 689,972 to 692,124 10721 mRNA_2354: 10722 - K01580 E4.1.1.15, gadB, gadA, GAD glutamate decarboxylase, at 692,257 to 694,787 10722
Group Condition mRNA_2350 mRNA_2351 mRNA_2352 mRNA_2353 mRNA_2354
10718 10719 10720 10721 10722
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -1.7 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -1.1 -0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.7 -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 -1.0 -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 -1.2 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 -1.1 +0.2 +0.3
auxotrophy YNB Glucose N.D. +0.4 -0.8 -0.1 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.4 -0.1 -0.4
auxotrophy Dropout Complete N.D. +0.4 -0.6 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.0 -0.2 +0.2
growth on oleic acid YNB Glucose N.D. -0.1 +0.5 +0.0 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.8 -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 +0.4 -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.5 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 +0.4 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 +1.3 -0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 +0.9 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 +1.2 -0.2 +0.2
growth on ypd YPD N.D. -0.4 +1.7 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +1.5 -0.4 +0.2
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