Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10720 and 10721 are separated by 410 nucleotides10721 and 10722 are separated by 132 nucleotides10722 and 10723 are separated by 67 nucleotides10723 and 10724 are separated by 857 nucleotides mRNA_2352: 10720 - K02903 RP-L28e, RPL28 large subunit ribosomal protein L28e, at 688,827 to 689,561 10720 mRNA_2353: 10721 - SUPERFAMILY--SSF88713, at 689,972 to 692,124 10721 mRNA_2354: 10722 - K01580 E4.1.1.15, gadB, gadA, GAD glutamate decarboxylase, at 692,257 to 694,787 10722 mRNA_2355: 10723 - Hypothetical Protein, at 694,855 to 695,610 10723 mRNA_2356: 10724 - Hypothetical Protein, at 696,468 to 697,388 10724
Group Condition mRNA_2352 mRNA_2353 mRNA_2354 mRNA_2355 mRNA_2356
10720 10721 10722 10723 10724
facs on lipid accumulation Sorted High BODIPY Gate -1.7 +0.1 +0.2 -0.2 +0.0
auxotrophy YNB Glucose -0.8 -0.1 +0.2 -0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine -1.0 -0.2 +0.2 -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine -1.2 +0.1 +0.1 -0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy -1.1 -0.1 -0.0 -0.2 +0.7
ypd buoyancy 40 hrs High Buoyancy -0.7 -0.1 +0.2 -0.2 +0.4
growth on ricinoleic acid YNB Glucose +0.4 -0.1 -0.4 +0.2 -0.5
growth on oleic acid YNB Glucose +0.5 +0.0 -0.1 -0.6 -0.2
auxotrophy Dropout Complete -0.6 +0.1 +0.0 +0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.0 -0.5 +0.4
growth on oleic acid YNB Oleic Acid +0.8 -0.1 -0.2 -0.6 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.2 +0.2 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.1 -0.1 +0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 +0.2 +0.3 +0.2 +0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.9 +0.1 +0.3 -0.6 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.2 +0.3 -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.1 +0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.3 -0.2 -0.2 -0.8 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.5 -0.4 +0.2 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.2 -0.2 +0.2 -0.0 +0.1
growth on ypd YPD +1.7 -0.1 +0.2 +0.7 -0.1
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