Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10728 and 10729 are separated by 421 nucleotides10729 and 10730 are separated by 1005 nucleotides10730 and 10731 are separated by 392 nucleotides10731 and 10732 are separated by 845 nucleotides mRNA_2360: 10728 - Hypothetical Protein, at 702,511 to 702,996 10728 mRNA_2361: 10729 - Hypothetical Protein, at 703,418 to 705,625 10729 mRNA_2362: 10730 - HMMPfam-Pentatricopeptide repeat domain-PF13812, at 706,631 to 709,566 10730 mRNA_2363: 10731 - KOG0017 FOG Transposon-encoded proteins with TYA, reverse transcriptase, integrase domains in various combinations, at 709,959 to 711,160 10731 mRNA_2364: 10732 - K20471 COPD, ARCN1, RET2 coatomer subunit delta, at 712,006 to 714,085 10732
Group Condition mRNA_2360 mRNA_2361 mRNA_2362 mRNA_2363 mRNA_2364
10728 10729 10730 10731 10732
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.4 N.D. -0.1 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.8 -0.2 N.D. +0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate -1.1 -0.3 N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy -1.0 -0.2 N.D. -0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -1.4 +0.5 N.D. +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 +0.1 N.D. -0.2 +0.2
growth on oleic acid YNB Glucose -0.6 +0.0 N.D. -0.2 +0.5
growth on ypd YPD -1.3 +0.4 N.D. +0.4 +0.4
growth on ricinoleic acid YNB Glucose +0.2 -0.4 N.D. +0.0 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 N.D. +0.0 +0.3
growth on oleic acid YNB Oleic Acid -0.2 +0.1 N.D. -0.6 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.5 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.3 N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.6 N.D. -0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.2 N.D. -0.1 +1.3
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.3 N.D. +0.0 +1.1
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.3 N.D. -0.2 +1.4
auxotrophy YNB Glucose +0.3 -0.1 N.D. -0.2 +1.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.0 N.D. +0.0 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.1 N.D. +0.4 +1.4
auxotrophy Dropout Complete +0.3 +0.1 N.D. +0.0 +1.8
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