Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10731 and 10732 are separated by 845 nucleotides10732 and 10733 are separated by 750 nucleotides10733 and 10734 are separated by 489 nucleotides10734 and 10735 are separated by 677 nucleotides mRNA_2363: 10731 - KOG0017 FOG Transposon-encoded proteins with TYA, reverse transcriptase, integrase domains in various combinations, at 709,959 to 711,160 10731 mRNA_2364: 10732 - K20471 COPD, ARCN1, RET2 coatomer subunit delta, at 712,006 to 714,085 10732 mRNA_2365: 10733 - HMMPfam-C2 domain-PF00168,HMMPfam-Munc13 (mammalian uncoordinated) homology domain-PF10540,ProSiteProfiles-Munc13-homology domain 1 (MHD1) profile.-PS51258,ProSiteProfiles-Munc13-homology domain 2 (MHD2) profile.-PS51259,SMART-Protein kinase C conserved region 2 (CalB)-SM00239,SUPERFAMILY--SSF49562, at 714,836 to 719,439 10733 mRNA_2366: 10734 - HMMPfam-GDP/GTP exchange factor Sec2p-PF06428,SUPERFAMILY--SSF144284, at 719,929 to 722,612 10734 mRNA_2367: 10735 - HMMPfam-Salt tolerance down-regulator-PF13945, at 723,290 to 728,071 10735
Group Condition mRNA_2363 mRNA_2364 mRNA_2365 mRNA_2366 mRNA_2367
10731 10732 10733 10734 10735
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +1.3 -2.6 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.2 -0.7 -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.7 -0.3 +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.0 +0.3 -1.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +1.4 -2.5 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.6 -1.2 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.0 -0.4 -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.2 -0.6 +0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.3 -0.5 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.9 -1.0 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 -0.8 +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.2 -0.6 +0.4 N.D.
auxotrophy YNB Glucose -0.2 +1.0 -0.7 +0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.6 +0.9 -0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.6 -0.8 +0.3 N.D.
growth on oleic acid YNB Glucose -0.2 +0.5 -0.3 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.1 -0.0 +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 +1.4 -0.6 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 +1.1 -0.4 +0.0 N.D.
growth on ypd YPD +0.4 +0.4 +0.5 -0.0 N.D.
auxotrophy Dropout Complete +0.0 +1.8 +0.0 -0.1 N.D.
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