Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10741 and 10742 are separated by 1216 nucleotides10742 and 10743 are separated by 241 nucleotides10743 and 10744 are separated by 259 nucleotides10744 and 10745 are separated by 321 nucleotides mRNA_2373: 10741 - K08286 E2.7.11.- protein-serine/threonine kinase, at 740,603 to 745,355 10741 mRNA_2374: 10742 - K12897 TRA2 transformer-2 protein, at 746,572 to 748,318 10742 mRNA_2375: 10743 - Hypothetical Protein, at 748,560 to 749,428 10743 mRNA_2376: 10744 - Hypothetical Protein, at 749,688 to 751,493 10744 mRNA_2377: 10745 - KOG0725 Reductases with broad range of substrate specificities, at 751,815 to 752,999 10745
Group Condition mRNA_2373 mRNA_2374 mRNA_2375 mRNA_2376 mRNA_2377
10741 10742 10743 10744 10745
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. -0.7 +0.3 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. -0.4 -0.7 -0.1
growth on ypd YPD -0.0 N.D. -0.3 -0.6 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. -0.5 +0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. +0.0 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.5 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.3 -0.3 +0.2
growth on oleic acid YNB Glucose +0.4 N.D. -0.2 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.1 -0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.4 -0.5 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.1 -0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.4 -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.0 -0.4 +0.5
growth on oleic acid YNB Oleic Acid +0.7 N.D. -0.1 +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. +0.4 -0.1 -0.4
auxotrophy Dropout Complete +0.5 N.D. +0.7 -0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +1.4 N.D. -0.2 -0.3 -0.3
growth on ricinoleic acid YNB Glucose +0.8 N.D. +0.3 -0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +1.5 N.D. -0.1 -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +1.2 -0.0 -0.5
auxotrophy YNB Glucose +0.8 N.D. +1.1 -0.2 -0.4
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