Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10749 and 10750 are separated by 130 nucleotides10750 and 10751 are separated by 590 nucleotides10751 and 10752 are separated by 19 nucleotides10752 and 10753 are separated by 279 nucleotides mRNA_2381: 10749 - KOG4660 Protein Mei2, essential for commitment to meiosis, and related proteins, at 757,956 to 760,560 10749 mRNA_2382: 10750 - HMMPfam-Zinc-binding loop region of homing endonuclease-PF05551,SUPERFAMILY--SSF54060, at 760,691 to 762,074 10750 mRNA_2383: 10751 - Hypothetical Protein, at 762,665 to 764,140 10751 mRNA_2384: 10752 - K11600 RRP41, EXOSC4, SKI6 exosome complex component RRP41, at 764,160 to 765,511 10752 mRNA_2385: 10753 - K01885 EARS, gltX glutamyl-tRNA synthetase, at 765,791 to 769,298 10753
Group Condition mRNA_2381 mRNA_2382 mRNA_2383 mRNA_2384 mRNA_2385
10749 10750 10751 10752 10753
facs on lipid accumulation Sorted High BODIPY Gate -2.5 N.D. +0.4 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -2.4 N.D. +0.4 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 N.D. -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 N.D. -0.6 N.D. N.D.
auxotrophy Dropout Complete -1.1 N.D. -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 N.D. -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. -0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.0 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 N.D. N.D.
auxotrophy YNB Glucose -0.0 N.D. -0.0 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.0 N.D. N.D.
growth on ypd YPD +0.3 N.D. -0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. -0.2 N.D. N.D.
growth on oleic acid YNB Glucose +0.7 N.D. -0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. -0.0 N.D. N.D.
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