Fitness for 4 genes in Rhodosporidium toruloides IFO0880

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500 nt 10752 and 10753 are separated by 279 nucleotides10753 and 10754 are separated by 471 nucleotides10754 and 10755 are separated by 196 nucleotides mRNA_2384: 10752 - K11600 RRP41, EXOSC4, SKI6 exosome complex component RRP41, at 764,160 to 765,511 10752 mRNA_2385: 10753 - K01885 EARS, gltX glutamyl-tRNA synthetase, at 765,791 to 769,298 10753 mRNA_2386: 10754 - K01530 E3.6.3.1 phospholipid-translocating ATPase, at 769,770 to 774,280 10754 mRNA_2387: 10755 - K17781 TIM13 mitochondrial import inner membrane translocase subunit TIM13, at 774,477 to 775,750 10755
Group Condition mRNA_2384 mRNA_2385 mRNA_2386 mRNA_2387
10752 10753 10754 10755
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -0.7
growth on ypd YPD N.D. N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.5 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.5 -0.3
growth on oleic acid YNB Glucose N.D. N.D. +0.6 -0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.6 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.5 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +1.0 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +1.2 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.4 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +1.3 -0.4
auxotrophy Dropout Complete N.D. N.D. +1.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +1.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.4 +0.6
auxotrophy YNB Glucose N.D. N.D. +1.4 -0.1
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