Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10756 and 10757 are separated by 900 nucleotides10757 and 10758 are separated by 709 nucleotides10758 and 10759 are separated by 324 nucleotides10759 and 10760 are separated by 309 nucleotides mRNA_2388: 10756 - K14781 DHR2 ATP-dependent RNA helicase DHR2, at 595 to 3,686 10756 mRNA_2389: 10757 - K10901 BLM, RECQL3, SGS1 bloom syndrome protein, at 4,587 to 7,344 10757 mRNA_2390: 10758 - Hypothetical Protein, at 8,054 to 9,276 10758 mRNA_2391: 10759 - HMMPfam-Chaperone for protein-folding within the ER, fungal-PF10681, at 9,601 to 10,907 10759 mRNA_2392: 10760 - K10364 CAPZA capping protein (actin filament) muscle Z-line, alpha, at 11,217 to 13,113 10760
Group Condition mRNA_2388 mRNA_2389 mRNA_2390 mRNA_2391 mRNA_2392
10756 10757 10758 10759 10760
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.3 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.3 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 -0.4 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 -0.0 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.0 -0.5 N.D. +0.4
auxotrophy Dropout Complete N.D. -0.4 -0.0 N.D. +0.3
growth on oleic acid YNB Glucose N.D. -0.1 -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 +0.4 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 -0.1 N.D. +0.0
growth on ypd YPD N.D. +0.1 +0.5 N.D. -0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.2 N.D. -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.5 N.D. +0.7
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 +0.2 N.D. +0.1
auxotrophy YNB Glucose N.D. -0.2 +0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -0.5 N.D. +0.7
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 +0.3 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 +0.1 N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 -0.4 N.D. +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 +0.5 N.D. -0.2
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