Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10800 and 10801 are separated by 157 nucleotides10801 and 10802 are separated by 723 nucleotides10802 and 10803 are separated by 967 nucleotides10803 and 10804 are separated by 276 nucleotides mRNA_2432: 10800 - K20028 ZDHHC2_15_20 palmitoyltransferase ZDHHC2/15/20, at 111,348 to 112,998 10800 mRNA_2433: 10801 - K10866 RAD50 DNA repair protein RAD50, at 113,156 to 118,783 10801 mRNA_2434: 10802 - HMMPfam-ATP synthase subunit H-PF05493, at 119,507 to 119,841 10802 mRNA_2435: 10803 - Hypothetical Protein, at 120,809 to 121,330 10803 mRNA_2436: 10804 - KOG0254 Predicted transporter (major facilitator superfamily), at 121,607 to 125,074 10804
Group Condition mRNA_2432 mRNA_2433 mRNA_2434 mRNA_2435 mRNA_2436
10800 10801 10802 10803 10804
auxotrophy YNB Glucose 75mM Arginine -0.9 -1.0 N.D. -0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.9 N.D. -0.2 -0.2
auxotrophy Dropout Complete -0.9 -0.4 N.D. -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.3 N.D. +0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.4 +0.7 N.D. -0.5 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.6 -0.5 N.D. -0.2 +0.4
growth on ypd YPD +0.1 -1.0 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.5 +0.0 N.D. -0.2 -0.0
auxotrophy YNB Glucose -0.5 -0.3 N.D. -0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.0 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 N.D. -0.0 -0.3
growth on oleic acid YNB Glucose -0.0 +0.0 N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.2 N.D. +0.3 -0.2
growth on oleic acid YNB Oleic Acid -0.2 +0.4 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.2 N.D. +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.3 N.D. -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.7 N.D. -0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.0 N.D. +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.0 N.D. -0.0 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.5 N.D. +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.6 N.D. -0.0 -0.1
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