Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10806 and 10807 are separated by 213 nucleotides10807 and 10808 are separated by 420 nucleotides10808 and 10809 are separated by 418 nucleotides10809 and 10810 are separated by 415 nucleotides mRNA_2438: 10806 - HMMPfam-Predicted coiled-coil protein (DUF2205)-PF10224, at 131,945 to 132,522 10806 mRNA_2439: 10807 - BLAST ribosome biogenesis protein [Moniliophthora rorer..., at 132,736 to 133,822 10807 mRNA_2440: 10808 - Hypothetical Protein, at 134,243 to 137,592 10808 mRNA_2441: 10809 - KOG1284 Bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2butanone-4-phosphate synthase, at 138,011 to 140,441 10809 mRNA_2442: 10810 - HMMPfam-ACT domain-PF13840,SUPERFAMILY--SSF55021, at 140,857 to 142,808 10810
Group Condition mRNA_2438 mRNA_2439 mRNA_2440 mRNA_2441 mRNA_2442
10806 10807 10808 10809 10810
facs on lipid accumulation Sorted Low BODIPY Gate -2.0 N.D. -0.4 -2.2 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -1.2 N.D. -0.4 -0.8 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -2.0 N.D. +0.1 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. +0.1 -1.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -1.0 N.D. -0.1 -0.2 +0.0
growth on ypd YPD -0.6 N.D. -0.1 +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. -0.2 +0.2 -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. -0.2 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.2 +0.0 -0.3
auxotrophy YNB Glucose +0.2 N.D. +0.0 -0.3 -0.0
growth on oleic acid YNB Oleic Acid +1.0 N.D. -0.4 +0.3 -0.8
ypd buoyancy 40 hrs Low Buoyancy +0.5 N.D. -0.4 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.2 +0.1 +0.4
auxotrophy Dropout Complete +0.5 N.D. -0.1 +0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.1 +0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +1.1 N.D. -0.4 +0.4 -0.5
growth on ricinoleic acid YNB Glucose +1.0 N.D. -0.3 +0.3 -0.4
growth on oleic acid YNB Glucose +0.8 N.D. -0.2 +0.6 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 N.D. +0.0 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.4 N.D. -0.0 +0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.4 N.D. -0.0 +1.0 -0.2
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