Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10808 and 10809 are separated by 418 nucleotides10809 and 10810 are separated by 415 nucleotides10810 and 10811 are separated by 538 nucleotides10811 and 10812 are separated by 351 nucleotides mRNA_2440: 10808 - Hypothetical Protein, at 134,243 to 137,592 10808 mRNA_2441: 10809 - KOG1284 Bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2butanone-4-phosphate synthase, at 138,011 to 140,441 10809 mRNA_2442: 10810 - HMMPfam-ACT domain-PF13840,SUPERFAMILY--SSF55021, at 140,857 to 142,808 10810 mRNA_2443: 10811 - HMMPfam-Peroxidase-PF00141,PRINTS-Haem peroxidase superfamily signature-PR00458,PRINTS-Plant ascorbate peroxidase signature-PR00459,ProSitePatterns-Peroxidases proximal heme-ligand signature.-PS00435,ProSitePatterns-Peroxidases active site signature.-PS00436,ProSiteProfiles-Plant heme peroxidase family profile.-PS50873,SUPERFAMILY--SSF48113, at 143,347 to 145,508 10811 mRNA_2444: 10812 - KOG4505 Na+/H+ antiporter, at 145,860 to 150,269 10812
Group Condition mRNA_2440 mRNA_2441 mRNA_2442 mRNA_2443 mRNA_2444
10808 10809 10810 10811 10812
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -2.2 -0.5 -0.5 -4.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -1.4 +0.1 -0.2 -2.3
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -0.8 -0.0 -0.0 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 -0.6 -1.4 -0.9
growth on oleic acid YNB Oleic Acid -0.4 +0.3 -0.8 -1.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.4 -0.5 -0.8 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.2 -0.1 +0.0 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 -0.1 -0.7 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.0 -0.3 -0.1 +0.1
growth on oleic acid YNB Glucose -0.2 +0.6 -0.5 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.2 +0.4 +0.0 -1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.0 +0.4 -0.3 -0.5
growth on ypd YPD -0.1 +0.0 -0.1 -0.4 +0.3
auxotrophy Dropout Complete -0.1 +0.2 -0.2 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.2 +0.0 +0.0 +0.1
growth on ricinoleic acid YNB Glucose -0.3 +0.3 -0.4 -0.2 +0.6
auxotrophy YNB Glucose +0.0 -0.3 -0.0 +0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 +0.1 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.1 +0.4 +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +1.0 -0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.5 +0.1 -0.1 +0.4
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