Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10809 and 10810 are separated by 415 nucleotides10810 and 10811 are separated by 538 nucleotides10811 and 10812 are separated by 351 nucleotides10812 and 10813 are separated by 1096 nucleotides mRNA_2441: 10809 - KOG1284 Bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2butanone-4-phosphate synthase, at 138,011 to 140,441 10809 mRNA_2442: 10810 - HMMPfam-ACT domain-PF13840,SUPERFAMILY--SSF55021, at 140,857 to 142,808 10810 mRNA_2443: 10811 - HMMPfam-Peroxidase-PF00141,PRINTS-Haem peroxidase superfamily signature-PR00458,PRINTS-Plant ascorbate peroxidase signature-PR00459,ProSitePatterns-Peroxidases proximal heme-ligand signature.-PS00435,ProSitePatterns-Peroxidases active site signature.-PS00436,ProSiteProfiles-Plant heme peroxidase family profile.-PS50873,SUPERFAMILY--SSF48113, at 143,347 to 145,508 10811 mRNA_2444: 10812 - KOG4505 Na+/H+ antiporter, at 145,860 to 150,269 10812 mRNA_2445: 10813 - HMMPfam-Protein of unknown function (DUF3533)-PF12051, at 151,366 to 153,133 10813
Group Condition mRNA_2441 mRNA_2442 mRNA_2443 mRNA_2444 mRNA_2445
10809 10810 10811 10812 10813
facs on lipid accumulation Sorted Low BODIPY Gate -2.2 -0.5 -0.5 -4.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -1.4 +0.1 -0.2 -2.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.0 -0.0 -1.9 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.6 -1.4 -0.9 -0.1
growth on oleic acid YNB Oleic Acid +0.3 -0.8 -1.0 -0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.1 -0.7 +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.5 -0.8 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 +0.0 -0.5 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.0 +0.0 +0.1 -0.5
growth on oleic acid YNB Glucose +0.6 -0.5 -0.2 -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.3 -0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.4 -0.3 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.4 +0.0 -1.1 +0.4
auxotrophy Dropout Complete +0.2 -0.2 -0.2 +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.1 +0.1 -0.3
growth on ricinoleic acid YNB Glucose +0.3 -0.4 -0.2 +0.6 -0.1
auxotrophy YNB Glucose -0.3 -0.0 +0.1 +0.4 +0.1
growth on ypd YPD +0.0 -0.1 -0.4 +0.3 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.4 +0.0 -0.1 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.1 -0.1 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 -0.2 -0.1 -0.1 +0.4
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