Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10819 and 10820 are separated by 259 nucleotides10820 and 10821 are separated by 468 nucleotides10821 and 10822 are separated by 985 nucleotides10822 and 10823 are separated by 138 nucleotides mRNA_2451: 10819 - Hypothetical Protein, at 163,388 to 164,949 10819 mRNA_2452: 10820 - HMMPfam-Ribonuclease H2 non-catalytic subunit (Ylr154p-like)-PF08615, at 165,209 to 166,286 10820 mRNA_2453: 10821 - HMMPfam-Fungal cellulose binding domain-PF00734,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF57180, at 166,755 to 168,701 10821 mRNA_2454: 10822 - K12823 DDX5, DBP2 ATP-dependent RNA helicase DDX5/DBP2, at 169,687 to 172,594 10822 mRNA_2455: 10823 - K16912 LAS1 ribosomal biogenesis protein LAS1, at 172,733 to 174,671 10823
Group Condition mRNA_2451 mRNA_2452 mRNA_2453 mRNA_2454 mRNA_2455
10819 10820 10821 10822 10823
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.2 -0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.8 -0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.4 +0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.5 +0.4 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.2 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.6 +0.3 -0.2 N.D. N.D.
growth on oleic acid YNB Glucose -0.5 +0.2 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.7 +0.5 -0.1 N.D. N.D.
auxotrophy Dropout Complete -0.2 -0.3 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 +0.6 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.2 -0.1 N.D. N.D.
growth on ypd YPD +0.2 -0.3 +0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.7 +0.5 +0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.3 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.3 -0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 -0.3 -0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.7 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.4 -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 -0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.4 +0.1 N.D. N.D.
auxotrophy YNB Glucose +0.1 +0.4 +0.3 N.D. N.D.
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