Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10820 and 10821 are separated by 468 nucleotides10821 and 10822 are separated by 985 nucleotides10822 and 10823 are separated by 138 nucleotides10823 and 10824 are separated by 226 nucleotides mRNA_2452: 10820 - HMMPfam-Ribonuclease H2 non-catalytic subunit (Ylr154p-like)-PF08615, at 165,209 to 166,286 10820 mRNA_2453: 10821 - HMMPfam-Fungal cellulose binding domain-PF00734,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF57180, at 166,755 to 168,701 10821 mRNA_2454: 10822 - K12823 DDX5, DBP2 ATP-dependent RNA helicase DDX5/DBP2, at 169,687 to 172,594 10822 mRNA_2455: 10823 - K16912 LAS1 ribosomal biogenesis protein LAS1, at 172,733 to 174,671 10823 mRNA_2456: 10824 - Hypothetical Protein, at 174,898 to 175,908 10824
Group Condition mRNA_2452 mRNA_2453 mRNA_2454 mRNA_2455 mRNA_2456
10820 10821 10822 10823 10824
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 -0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.4 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.1 N.D. N.D. N.D.
growth on ypd YPD -0.3 +0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.4 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 N.D. N.D. N.D.
auxotrophy Dropout Complete -0.3 +0.3 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.2 -0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.3 -0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.0 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 -0.1 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 -0.1 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.4 +0.3 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.5 +0.2 N.D. N.D. N.D.
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