Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10821 and 10822 are separated by 985 nucleotides10822 and 10823 are separated by 138 nucleotides10823 and 10824 are separated by 226 nucleotides10824 and 10825 are separated by 1824 nucleotides mRNA_2453: 10821 - HMMPfam-Fungal cellulose binding domain-PF00734,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF57180, at 166,755 to 168,701 10821 mRNA_2454: 10822 - K12823 DDX5, DBP2 ATP-dependent RNA helicase DDX5/DBP2, at 169,687 to 172,594 10822 mRNA_2455: 10823 - K16912 LAS1 ribosomal biogenesis protein LAS1, at 172,733 to 174,671 10823 mRNA_2456: 10824 - Hypothetical Protein, at 174,898 to 175,908 10824 mRNA_2457: 10825 - K10589 UBE3C ubiquitin-protein ligase E3 C, at 177,733 to 180,454 10825
Group Condition mRNA_2453 mRNA_2454 mRNA_2455 mRNA_2456 mRNA_2457
10821 10822 10823 10824 10825
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. N.D. -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. N.D. -0.3
growth on oleic acid YNB Glucose -0.1 N.D. N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. N.D. -0.3
growth on ypd YPD +0.1 N.D. N.D. N.D. -0.2
auxotrophy YNB Glucose +0.3 N.D. N.D. N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.2 N.D. N.D. N.D. -0.1
auxotrophy Dropout Complete +0.3 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. N.D. +0.5
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