Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10822 and 10823 are separated by 138 nucleotides10823 and 10824 are separated by 226 nucleotides10824 and 10825 are separated by 1824 nucleotides10825 and 10826 are separated by 3330 nucleotides mRNA_2454: 10822 - K12823 DDX5, DBP2 ATP-dependent RNA helicase DDX5/DBP2, at 169,687 to 172,594 10822 mRNA_2455: 10823 - K16912 LAS1 ribosomal biogenesis protein LAS1, at 172,733 to 174,671 10823 mRNA_2456: 10824 - Hypothetical Protein, at 174,898 to 175,908 10824 mRNA_2457: 10825 - K10589 UBE3C ubiquitin-protein ligase E3 C, at 177,733 to 180,454 10825 mRNA_2458: 10826 - K04799 FEN1, RAD2 flap endonuclease-1, at 183,785 to 185,672 10826
Group Condition mRNA_2454 mRNA_2455 mRNA_2456 mRNA_2457 mRNA_2458
10822 10823 10824 10825 10826
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.4 N.D.
auxotrophy YNB Glucose N.D. N.D. N.D. -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.3 N.D.
growth on ypd YPD N.D. N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.1 N.D.
auxotrophy Dropout Complete N.D. N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.5 N.D.
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