Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10823 and 10824 are separated by 226 nucleotides10824 and 10825 are separated by 1824 nucleotides10825 and 10826 are separated by 3330 nucleotides10826 and 10827 are separated by 370 nucleotides mRNA_2455: 10823 - K16912 LAS1 ribosomal biogenesis protein LAS1, at 172,733 to 174,671 10823 mRNA_2456: 10824 - Hypothetical Protein, at 174,898 to 175,908 10824 mRNA_2457: 10825 - K10589 UBE3C ubiquitin-protein ligase E3 C, at 177,733 to 180,454 10825 mRNA_2458: 10826 - K04799 FEN1, RAD2 flap endonuclease-1, at 183,785 to 185,672 10826 mRNA_2459: 10827 - KOG2747 Histone acetyltransferase (MYST family), at 186,043 to 188,568 10827
Group Condition mRNA_2455 mRNA_2456 mRNA_2457 mRNA_2458 mRNA_2459
10823 10824 10825 10826 10827
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.5 N.D. -2.9
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.4 N.D. -2.8
auxotrophy Dropout Complete N.D. N.D. -0.1 N.D. -2.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 N.D. -1.9
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 N.D. -1.6
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.7 N.D. -0.9
auxotrophy YNB Glucose N.D. N.D. -0.4 N.D. -1.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 N.D. -0.6
growth on ypd YPD N.D. N.D. -0.2 N.D. -0.5
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.5 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 N.D. +0.7
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. +0.7
growth on oleic acid YNB Glucose N.D. N.D. -0.2 N.D. +0.7
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.0 N.D. +1.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.0 N.D. +1.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. +1.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 N.D. +2.0
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