Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10825 and 10826 are separated by 3330 nucleotides10826 and 10827 are separated by 370 nucleotides10827 and 10828 are separated by 377 nucleotides10828 and 10829 are separated by 138 nucleotides mRNA_2457: 10825 - K10589 UBE3C ubiquitin-protein ligase E3 C, at 177,733 to 180,454 10825 mRNA_2458: 10826 - K04799 FEN1, RAD2 flap endonuclease-1, at 183,785 to 185,672 10826 mRNA_2459: 10827 - KOG2747 Histone acetyltransferase (MYST family), at 186,043 to 188,568 10827 mRNA_2460: 10828 - SUPERFAMILY--SSF57997, at 188,946 to 195,534 10828 mRNA_2461: 10829 - Hypothetical Protein, at 195,673 to 198,074 10829
Group Condition mRNA_2457 mRNA_2458 mRNA_2459 mRNA_2460 mRNA_2461
10825 10826 10827 10828 10829
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -2.8 -0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. -2.9 -0.1 +0.4
auxotrophy Dropout Complete -0.1 N.D. -2.3 -0.3 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -1.9 -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. -0.9 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -1.6 -0.1 +0.3
auxotrophy YNB Glucose -0.4 N.D. -1.0 -0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.6 -0.1 -0.1
growth on ypd YPD -0.2 N.D. -0.5 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.2 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.5 N.D. -0.3 -0.0 -0.3
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.1 -0.0 +0.2
growth on oleic acid YNB Glucose -0.2 N.D. +0.7 -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.6 +0.0 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. +0.7 +0.0 +0.0
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.4 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. +0.7 -0.0 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. +1.5 +0.0 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. +1.5 -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +1.7 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. +2.0 +0.0 +0.5
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