Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10836 and 10837 are separated by 274 nucleotides10837 and 10838 are separated by 406 nucleotides10838 and 10839 are separated by 2047 nucleotides10839 and 10840 are separated by 513 nucleotides mRNA_2468: 10836 - HMMPfam-Protein of unknown function (DUF3605)-PF12239, at 219,439 to 220,603 10836 mRNA_2469: 10837 - K12479 VPS45 vacuolar protein sorting-associated protein 45, at 220,878 to 223,726 10837 mRNA_2470: 10838 - K06659 CLB3_4 G2/mitotic-specific cyclin 3/4, at 224,133 to 225,151 10838 mRNA_2471: 10839 - K13104 ZNF830, CCDC16 zinc finger protein 830, at 227,199 to 228,171 10839 mRNA_2472: 10840 - K07836 RAP1B Ras-related protein Rap-1B, at 228,685 to 229,666 10840
Group Condition mRNA_2468 mRNA_2469 mRNA_2470 mRNA_2471 mRNA_2472
10836 10837 10838 10839 10840
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 N.D. N.D. -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. -0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.2 N.D. N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. N.D. -0.7 N.D.
auxotrophy Dropout Complete -0.0 N.D. N.D. -0.2 N.D.
growth on ypd YPD -0.1 N.D. N.D. -0.0 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. N.D. -0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. N.D. -0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. N.D. -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.5 N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. N.D. +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.6 N.D. N.D. -0.1 N.D.
auxotrophy YNB Glucose +0.4 N.D. N.D. +0.2 N.D.
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