Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10837 and 10838 are separated by 406 nucleotides10838 and 10839 are separated by 2047 nucleotides10839 and 10840 are separated by 513 nucleotides10840 and 10841 are separated by 469 nucleotides mRNA_2469: 10837 - K12479 VPS45 vacuolar protein sorting-associated protein 45, at 220,878 to 223,726 10837 mRNA_2470: 10838 - K06659 CLB3_4 G2/mitotic-specific cyclin 3/4, at 224,133 to 225,151 10838 mRNA_2471: 10839 - K13104 ZNF830, CCDC16 zinc finger protein 830, at 227,199 to 228,171 10839 mRNA_2472: 10840 - K07836 RAP1B Ras-related protein Rap-1B, at 228,685 to 229,666 10840 mRNA_2473: 10841 - K01610 E4.1.1.49, pckA phosphoenolpyruvate carboxykinase (ATP), at 230,136 to 233,558 10841
Group Condition mRNA_2469 mRNA_2470 mRNA_2471 mRNA_2472 mRNA_2473
10837 10838 10839 10840 10841
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 N.D. -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.2 N.D. -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.7 N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 N.D. -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.5 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.6 N.D. +0.0
auxotrophy Dropout Complete N.D. N.D. -0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.3 N.D. -0.2
growth on ypd YPD N.D. N.D. -0.0 N.D. -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.0 N.D. -0.1
auxotrophy YNB Glucose N.D. N.D. +0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.0 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 N.D. +0.2
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