Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10842 and 10843 are separated by 321 nucleotides10843 and 10844 are separated by 1578 nucleotides10844 and 10845 are separated by 417 nucleotides10845 and 10846 are separated by 380 nucleotides mRNA_2474: 10842 - KOG0603 Ribosomal protein S6 kinase, at 234,258 to 235,956 10842 mRNA_2475: 10843 - BLAST CAMK protein kinase [Microbotryum lychnidis-dioicae p..., at 236,278 to 238,719 10843 mRNA_2476: 10844 - KOG0673 Thymidylate synthase, at 240,298 to 241,620 10844 mRNA_2477: 10845 - K00560 thyA, TYMS thymidylate synthase, at 242,038 to 243,139 10845 mRNA_2478: 10846 - K20293 COG6, COD2 conserved oligomeric Golgi complex subunit 6, at 243,520 to 246,165 10846
Group Condition mRNA_2474 mRNA_2475 mRNA_2476 mRNA_2477 mRNA_2478
10842 10843 10844 10845 10846
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.4 -1.0 N.D. -0.2
growth on ypd YPD -0.2 -0.1 -0.1 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.2 -0.5 N.D. -0.1
growth on oleic acid YNB Glucose -0.3 -0.2 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.5 -0.5 N.D. +0.9
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.4 +0.2 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 -0.2 N.D. -0.3
growth on oleic acid YNB Oleic Acid -0.5 -0.4 +0.2 N.D. +0.4
auxotrophy Dropout Complete -0.1 -0.1 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.1 -0.5 +0.1 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.3 -0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.5 -0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.2 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.4 -0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.4 -0.3 N.D. +1.3
auxotrophy YNB Glucose +0.1 +0.4 -0.1 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.4 +0.5 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.1 +0.6 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.2 +0.7 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.3 +0.6 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.0 +0.6 N.D. +1.0
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