Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10844 and 10845 are separated by 417 nucleotides10845 and 10846 are separated by 380 nucleotides10846 and 10847 are separated by 690 nucleotides10847 and 10848 are separated by 77 nucleotides mRNA_2476: 10844 - KOG0673 Thymidylate synthase, at 240,298 to 241,620 10844 mRNA_2477: 10845 - K00560 thyA, TYMS thymidylate synthase, at 242,038 to 243,139 10845 mRNA_2478: 10846 - K20293 COG6, COD2 conserved oligomeric Golgi complex subunit 6, at 243,520 to 246,165 10846 mRNA_2479: 10847 - Hypothetical Protein, at 246,856 to 249,133 10847 mRNA_2480: 10848 - KOG0907 Thioredoxin, at 249,211 to 249,707 10848
Group Condition mRNA_2476 mRNA_2477 mRNA_2478 mRNA_2479 mRNA_2480
10844 10845 10846 10847 10848
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. -0.1 -0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.3 -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 N.D. -0.2 +0.6 +0.2
growth on oleic acid YNB Glucose -0.2 N.D. +0.2 -0.0 -0.4
growth on ypd YPD -0.1 N.D. -0.5 +0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. +0.9 +0.1 -0.6
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.4 -0.1 -0.7
auxotrophy Dropout Complete -0.2 N.D. +0.2 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.3 -0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.1 -0.1 +0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.4 +0.0 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.2 +0.3 +0.1
auxotrophy YNB Glucose -0.1 N.D. +0.3 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. +1.3 -0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.1 +0.2 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.3 +0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.1 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. +0.2 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. +0.5 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. +0.7 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. +1.0 +0.1 -0.2
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