Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10846 and 10847 are separated by 690 nucleotides10847 and 10848 are separated by 77 nucleotides10848 and 10849 are separated by 329 nucleotides10849 and 10850 are separated by 1696 nucleotides mRNA_2478: 10846 - K20293 COG6, COD2 conserved oligomeric Golgi complex subunit 6, at 243,520 to 246,165 10846 mRNA_2479: 10847 - Hypothetical Protein, at 246,856 to 249,133 10847 mRNA_2480: 10848 - KOG0907 Thioredoxin, at 249,211 to 249,707 10848 mRNA_2481: 10849 - Hypothetical Protein, at 250,037 to 250,801 10849 mRNA_2482: 10850 - KOG0712 Molecular chaperone (DnaJ superfamily), at 252,498 to 254,224 10850
Group Condition mRNA_2478 mRNA_2479 mRNA_2480 mRNA_2481 mRNA_2482
10846 10847 10848 10849 10850
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.2 +0.1 -0.1 -0.5
growth on oleic acid YNB Oleic Acid +0.4 -0.1 -0.7 -0.2 -0.1
growth on ypd YPD -0.5 +0.1 +0.2 -0.2 -0.2
growth on oleic acid YNB Glucose +0.2 -0.0 -0.4 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 +0.1 -0.6 -0.4 -0.3
growth on ricinoleic acid YNB Glucose +0.4 +0.0 -0.4 -0.1 -0.1
auxotrophy Dropout Complete +0.2 +0.2 -0.2 -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.0 +0.4 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.1 -0.0 -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 +0.1 +0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.6 +0.2 +0.0 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.3 +0.1 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 -0.0 +0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +1.3 -0.2 -0.3 +0.0 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.1 -0.3 -0.1 +0.1
auxotrophy YNB Glucose +0.3 +0.2 +0.1 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 +0.3 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 +0.2 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.1 -0.1 +0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.2 +0.6 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 +0.1 -0.2 +0.1 +0.1
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