Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10852 and 10853 are separated by 87 nucleotides10853 and 10854 are separated by 391 nucleotides10854 and 10855 are separated by 525 nucleotides10855 and 10856 are separated by 1507 nucleotides mRNA_2484: 10852 - K11498 CENPE centromeric protein E, at 257,699 to 260,992 10852 mRNA_2485: 10853 - K07760 CDK cyclin-dependent kinase, at 261,080 to 262,482 10853 mRNA_2486: 10854 - KOG3134 Predicted membrane protein, at 262,874 to 263,661 10854 mRNA_2487: 10855 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 264,187 to 266,262 10855 mRNA_2488: 10856 - KOG1804 RNA helicase, at 267,770 to 269,827 10856
Group Condition mRNA_2484 mRNA_2485 mRNA_2486 mRNA_2487 mRNA_2488
10852 10853 10854 10855 10856
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -2.4 -1.0 -0.9 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -2.8 -0.2 -0.5 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -2.8 -0.0 +0.2 -0.8
growth on oleic acid YNB Oleic Acid -0.2 -2.0 -0.3 -0.6 -0.2
growth on ricinoleic acid YNB Glucose -0.2 -1.5 -0.2 -0.6 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -1.3 +0.2 -0.5 -0.2
growth on ypd YPD +0.1 -1.2 -0.7 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -1.5 -0.1 +0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.6 -0.3 -0.4 +0.4
growth on oleic acid YNB Glucose -0.1 -0.3 -0.1 -0.5 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 -0.1 -0.3 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.4 -0.4 +0.2 -0.2
auxotrophy YNB Glucose -0.4 -0.8 +0.3 +0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.6 -0.2 +0.5 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -1.4 -0.0 +0.8 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.2 -0.0 -0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.0 -0.3 +0.1 +0.2
auxotrophy Dropout Complete -0.3 -0.3 -0.2 +1.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.0 +0.3 +0.8 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.8 -0.3 +0.6 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 +1.3 -0.0 -0.0 +0.2
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