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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10855 and 10856 are separated by 1507 nucleotides
10856 and 10857 are separated by 393 nucleotides
10857 and 10858 are separated by 658 nucleotides
10858 and 10859 are separated by 202 nucleotides
mRNA_2487: 10855 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 264,187 to 266,262
10855
mRNA_2488: 10856 - KOG1804 RNA helicase, at 267,770 to 269,827
10856
mRNA_2489: 10857 - HMMPfam-Surfeit locus protein 5 subunit 22 of Mediator complex-PF06179, at 270,221 to 270,948
10857
mRNA_2490: 10858 - K14297 NUP98, ADAR2, NUP116 nuclear pore complex protein Nup98-Nup96, at 271,607 to 274,886
10858
mRNA_2491: 10859 - K12837 U2AF2 splicing factor U2AF 65 kDa subunit, at 275,089 to 277,521
10859
Group
Condition
mRNA
_2487
mRNA
_2488
mRNA
_2489
mRNA
_2490
mRNA
_2491
10855
10856
10857
10858
10859
facs on lipid accumulation
Sorted No BODIPY Gate
-0.9
-0.5
N.D.
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
-0.6
-0.2
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.5
-0.2
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
-0.8
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
-0.6
+0.0
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.5
-0.1
N.D.
N.D.
N.D.
growth on oleic acid
YNB Glucose
-0.5
+0.0
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.3
+0.0
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
+0.0
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.4
+0.4
N.D.
N.D.
N.D.
growth on ypd
YPD
-0.1
+0.1
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
-0.2
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.3
-0.1
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.0
+0.2
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose
+0.3
+0.0
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.2
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
+0.8
-0.0
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
+0.6
+0.2
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
+0.5
+0.3
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
+0.8
+0.2
N.D.
N.D.
N.D.
auxotrophy
Dropout Complete
+1.1
-0.0
N.D.
N.D.
N.D.
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