Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10855 and 10856 are separated by 1507 nucleotides10856 and 10857 are separated by 393 nucleotides10857 and 10858 are separated by 658 nucleotides10858 and 10859 are separated by 202 nucleotides mRNA_2487: 10855 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 264,187 to 266,262 10855 mRNA_2488: 10856 - KOG1804 RNA helicase, at 267,770 to 269,827 10856 mRNA_2489: 10857 - HMMPfam-Surfeit locus protein 5 subunit 22 of Mediator complex-PF06179, at 270,221 to 270,948 10857 mRNA_2490: 10858 - K14297 NUP98, ADAR2, NUP116 nuclear pore complex protein Nup98-Nup96, at 271,607 to 274,886 10858 mRNA_2491: 10859 - K12837 U2AF2 splicing factor U2AF 65 kDa subunit, at 275,089 to 277,521 10859
Group Condition mRNA_2487 mRNA_2488 mRNA_2489 mRNA_2490 mRNA_2491
10855 10856 10857 10858 10859
facs on lipid accumulation Sorted No BODIPY Gate -0.9 -0.5 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.6 -0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.2 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.8 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.6 +0.0 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.1 N.D. N.D. N.D.
growth on oleic acid YNB Glucose -0.5 +0.0 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.0 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.4 N.D. N.D. N.D.
growth on ypd YPD -0.1 +0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.3 +0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.8 -0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 +0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.3 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.2 N.D. N.D. N.D.
auxotrophy Dropout Complete +1.1 -0.0 N.D. N.D. N.D.
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