Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10859 and 10860 are separated by 239 nucleotides10860 and 10861 are separated by 891 nucleotides10861 and 10862 are separated by 545 nucleotides10862 and 10863 are separated by 321 nucleotides mRNA_2491: 10859 - K12837 U2AF2 splicing factor U2AF 65 kDa subunit, at 275,089 to 277,521 10859 mRNA_2492: 10860 - HMMPfam-Rsm1-like-PF08600, at 277,761 to 278,480 10860 mRNA_2493: 10861 - KOG3098 Uncharacterized conserved protein, at 279,372 to 281,338 10861 mRNA_2494: 10862 - HMMPfam-Taurine catabolism dioxygenase TauD, TfdA family-PF02668,SUPERFAMILY--SSF51197, at 281,884 to 283,232 10862 mRNA_2495: 10863 - K03507 DPB11 DNA replication regulator DPB11, at 283,554 to 287,606 10863
Group Condition mRNA_2491 mRNA_2492 mRNA_2493 mRNA_2494 mRNA_2495
10859 10860 10861 10862 10863
auxotrophy Dropout Complete N.D. -0.6 +0.1 -0.2 -1.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 +0.2 -0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.2 +0.1 -1.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 -0.1 +0.0 -0.4
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.1 -0.2 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.0 +0.3 -0.3 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 -0.2 +0.0 -0.1
auxotrophy YNB Glucose N.D. +0.0 +0.0 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 +0.0 -0.4 +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 +0.3 -0.1 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 +0.4 +0.1 -0.2
growth on ypd YPD N.D. +0.1 +0.1 +0.0 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 +0.3 -0.3 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 +0.1 -0.0 +0.5
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.2 -0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.1 +0.1 +0.3
growth on oleic acid YNB Glucose N.D. +0.2 -0.3 +0.1 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.0 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 +0.1 -0.4 +1.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.2 -0.1 +0.6
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