Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10869 and 10870 are separated by 250 nucleotides10870 and 10871 are separated by 527 nucleotides10871 and 10872 are separated by 1003 nucleotides10872 and 10873 are separated by 49 nucleotides mRNA_2501: 10869 - K00852 rbsK, RBKS ribokinase, at 301,795 to 303,224 10869 mRNA_2502: 10870 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 303,475 to 306,650 10870 mRNA_2503: 10871 - SUPERFAMILY--SSF63829, at 307,178 to 308,891 10871 mRNA_2504: 10872 - BLAST Immunoglobulin-like domain contaning protein [Rho..., at 309,895 to 311,581 10872 mRNA_2505: 10873 - KOG3299 Uncharacterized conserved protein, at 311,631 to 312,608 10873
Group Condition mRNA_2501 mRNA_2502 mRNA_2503 mRNA_2504 mRNA_2505
10869 10870 10871 10872 10873
auxotrophy Dropout Complete +0.2 -0.6 -0.2 -0.4 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.9 -0.0 -0.3 +0.2
growth on oleic acid YNB Oleic Acid +0.1 -0.6 -0.6 +0.0 -0.1
growth on oleic acid YNB Glucose -0.0 -0.6 -0.3 -0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.1 +0.7 +0.0 -1.1
ypd buoyancy 40 hrs Median Buoyancy -0.5 -0.4 -0.4 +0.0 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.7 +0.2 +0.2 -0.4
auxotrophy YNB Glucose +0.0 -0.7 +0.1 -0.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 +0.0 +0.7 -0.0 -0.7
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 -0.6 -0.0 +0.4
growth on ypd YPD +0.2 -0.1 -0.3 -0.2 +0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.8 +0.2 +0.1 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.6 -0.4 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.3 -0.0 -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 +0.3 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 -0.1 -0.0 +0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.3 +0.2 +0.1 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.9 +0.1 +0.0 +1.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.3 +0.4 +0.1 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.6 +0.3 +0.1 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.7 +0.8 +0.1 +0.9
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