Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10873 and 10874 overlap by 2 nucleotides10874 and 10875 are separated by 648 nucleotides10875 and 10876 are separated by 904 nucleotides10876 and 10877 are separated by 432 nucleotides mRNA_2505: 10873 - KOG3299 Uncharacterized conserved protein, at 311,631 to 312,608 10873 mRNA_2506: 10874 - HMMPfam-GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p-PF13839, at 312,607 to 314,311 10874 mRNA_2507: 10875 - K11232 YPD1 osomolarity two-component system, phosphorelay intermediate protein YPD1, at 314,960 to 315,808 10875 mRNA_2508: 10876 - BLAST proteophosphoglycan ppg4 [Lichtheimia corymbifera JMR..., at 316,713 to 320,410 10876 mRNA_2509: 10877 - HMMPfam-PI31 proteasome regulator-PF08577,HMMPfam-PI31 proteasome regulator N-terminal-PF11566, at 320,843 to 322,229 10877
Group Condition mRNA_2505 mRNA_2506 mRNA_2507 mRNA_2508 mRNA_2509
10873 10874 10875 10876 10877
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 +0.4 N.D. -0.5 -0.1
auxotrophy Dropout Complete -0.5 +0.0 N.D. -0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.0 N.D. -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -1.1 -0.1 N.D. +0.1 +0.9
growth on oleic acid YNB Glucose +0.0 +0.1 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 -0.1 N.D. +0.1 -0.8
ypd buoyancy 40 hrs Low Buoyancy +0.7 -0.2 N.D. +0.2 -0.6
auxotrophy YNB Glucose +0.0 +0.1 N.D. -0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.1 N.D. -0.1 -0.0
growth on oleic acid YNB Oleic Acid -0.1 +0.1 N.D. +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.0 N.D. -0.2 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.0 N.D. +0.0 -0.1
growth on ypd YPD +0.1 +0.1 N.D. -0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.5 -0.1 N.D. -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 N.D. +0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.1 N.D. +0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +1.0 -0.3 N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 N.D. -0.1 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.4 -0.1 N.D. +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 -0.0 N.D. +0.0 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 -0.2 N.D. +0.3 +0.8
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