Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10885 and 10886 are separated by 144 nucleotides10886 and 10887 are separated by 1213 nucleotides10887 and 10888 are separated by 523 nucleotides10888 and 10889 are separated by 346 nucleotides mRNA_2517: 10885 - K01078 E3.1.3.2 acid phosphatase, at 337,169 to 339,877 10885 mRNA_2518: 10886 - K03189 ureG urease accessory protein, at 340,022 to 341,494 10886 mRNA_2519: 10887 - SMART-Parallel beta-helix repeats-SM00710,SUPERFAMILY--SSF51126, at 342,708 to 344,522 10887 mRNA_2520: 10888 - KOG1441 Glucose-6-phosphate/phosphate and phosphoenolpyruvate/phosphate antiporter, at 345,046 to 346,549 10888 mRNA_2521: 10889 - BLAST beta-glucosidase 1 precursor [Aspergillus rambellii], at 346,896 to 348,680 10889
Group Condition mRNA_2517 mRNA_2518 mRNA_2519 mRNA_2520 mRNA_2521
10885 10886 10887 10888 10889
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 +0.1 -0.1 -1.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.3 +0.5 -0.1 -2.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 -0.2 -0.2 -0.8
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 -0.3 -0.3 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.2 -0.2 +0.0 -0.3
growth on oleic acid YNB Oleic Acid -0.0 +0.1 -0.1 +0.5 -0.8
growth on ypd YPD -0.0 +0.4 +0.2 -0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.0 -0.0 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 +0.5 -0.2 -0.0 -0.3
growth on oleic acid YNB Glucose -0.1 -0.2 +0.1 +0.4 +0.5
auxotrophy YNB Glucose -0.0 +0.3 -0.2 +0.4 +0.3
auxotrophy Dropout Complete -0.0 +0.1 -0.1 +0.4 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 +0.1 -0.5 +1.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.2 -0.1 +0.4 +0.5
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.3 +0.1 +0.5 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.5 -0.2 +0.5 +0.4
growth on ricinoleic acid YNB Glucose +0.2 -0.1 +0.2 +0.4 +0.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.3 +0.2 +0.7 +0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.1 +0.3 +0.2 +1.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.2 +0.1 +0.1 +1.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.3 +0.4 +1.0 +0.9
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