Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10893 and 10894 are separated by 601 nucleotides10894 and 10895 are separated by 2871 nucleotides10895 and 10896 are separated by 385 nucleotides10896 and 10897 are separated by 159 nucleotides mRNA_2525: 10893 - KOG0590 Checkpoint kinase and related serine/threonine protein kinases, at 354,865 to 358,254 10893 mRNA_2526: 10894 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 358,856 to 360,952 10894 mRNA_2527: 10895 - BLAST protein DGCR14-like [Mucor ambiguus], at 363,824 to 364,696 10895 mRNA_2528: 10896 - HMMPfam-Fungal chitosanase of glycosyl hydrolase group 75-PF07335, at 365,082 to 366,780 10896 mRNA_2529: 10897 - K01613 psd, PISD phosphatidylserine decarboxylase, at 366,940 to 368,677 10897
Group Condition mRNA_2525 mRNA_2526 mRNA_2527 mRNA_2528 mRNA_2529
10893 10894 10895 10896 10897
growth on ypd YPD -0.1 +0.0 +0.4 -0.5 -0.2
auxotrophy Dropout Complete -0.1 +0.0 +0.1 +0.1 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +0.3 -0.4 +0.4 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.1 +0.0 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 -0.5 -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.0 +0.2 -0.3 +0.0
growth on ricinoleic acid YNB Glucose -0.1 +0.2 +0.4 +0.1 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.4 +0.4 -0.2 -0.4
growth on oleic acid YNB Glucose -0.0 +0.1 +0.1 +0.5 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 +0.7 +0.0 -0.2
growth on oleic acid YNB Oleic Acid +0.0 +0.3 +0.5 +0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.2 +0.5 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.0 -0.9 +1.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 +1.0 +0.1 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.3 +0.1 +0.3 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 +0.1 +0.8 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 +0.4 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 -0.2 +0.6 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 -0.3 +0.9 +0.5
auxotrophy YNB Glucose +0.0 +0.1 +0.9 +0.5 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.3 +0.9 +0.8 +0.2
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