Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10894 and 10895 are separated by 2871 nucleotides10895 and 10896 are separated by 385 nucleotides10896 and 10897 are separated by 159 nucleotides10897 and 10898 are separated by 272 nucleotides mRNA_2526: 10894 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 358,856 to 360,952 10894 mRNA_2527: 10895 - BLAST protein DGCR14-like [Mucor ambiguus], at 363,824 to 364,696 10895 mRNA_2528: 10896 - HMMPfam-Fungal chitosanase of glycosyl hydrolase group 75-PF07335, at 365,082 to 366,780 10896 mRNA_2529: 10897 - K01613 psd, PISD phosphatidylserine decarboxylase, at 366,940 to 368,677 10897 mRNA_2530: 10898 - K14634 TIGAR fructose-2,6-bisphosphatase, at 368,950 to 370,646 10898
Group Condition mRNA_2526 mRNA_2527 mRNA_2528 mRNA_2529 mRNA_2530
10894 10895 10896 10897 10898
auxotrophy Dropout Complete +0.0 +0.1 +0.1 -0.5 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.4 +0.4 -0.5 -0.3
growth on ypd YPD +0.0 +0.4 -0.5 -0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.4 -0.2 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.5 -0.0 +0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 -0.3 +0.0 +0.0
growth on ricinoleic acid YNB Glucose +0.2 +0.4 +0.1 -0.4 -0.0
growth on oleic acid YNB Oleic Acid +0.3 +0.5 +0.0 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.0 -0.1 +0.2 +0.1
growth on oleic acid YNB Glucose +0.1 +0.1 +0.5 -0.4 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 +1.0 +0.1 -0.5 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.7 +0.0 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 +0.8 -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.5 +0.1 -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.4 +0.5 -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.1 +0.3 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.9 +1.1 +0.5 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.3 +0.9 +0.5 +0.1
auxotrophy YNB Glucose +0.1 +0.9 +0.5 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.2 +0.6 +0.3 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.9 +0.8 +0.2 +0.1
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