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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10896 and 10897 are separated by 159 nucleotides
10897 and 10898 are separated by 272 nucleotides
10898 and 10899 are separated by 1622 nucleotides
10899 and 10900 are separated by 173 nucleotides
mRNA_2528: 10896 - HMMPfam-Fungal chitosanase of glycosyl hydrolase group 75-PF07335, at 365,082 to 366,780
10896
mRNA_2529: 10897 - K01613 psd, PISD phosphatidylserine decarboxylase, at 366,940 to 368,677
10897
mRNA_2530: 10898 - K14634 TIGAR fructose-2,6-bisphosphatase, at 368,950 to 370,646
10898
mRNA_2531: 10899 - KOG2816 Predicted transporter ADD1 (major facilitator superfamily), at 372,269 to 375,397
10899
mRNA_2532: 10900 - KOG2961 Predicted hydrolase (HAD superfamily), at 375,571 to 377,208
10900
Group
Condition
mRNA
_2528
mRNA
_2529
mRNA
_2530
mRNA
_2531
mRNA
_2532
10896
10897
10898
10899
10900
auxotrophy
Dropout Complete
+0.1
-0.5
-0.4
-0.2
-0.6
auxotrophy
YNB Glucose 75mM Methionine
+0.1
-0.1
-0.2
-0.1
-1.1
growth on oleic acid
YNB Oleic Acid
+0.0
-0.3
-0.3
-0.2
-0.4
ypd buoyancy
40 hrs Low Buoyancy
+0.1
-0.5
-0.1
+0.2
-0.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.2
-0.4
-0.2
+0.6
-0.5
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
+0.2
+0.1
+0.1
-0.8
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.0
+0.1
+0.3
-0.4
-0.4
auxotrophy
YNB Glucose 75mM Arginine
+0.5
-0.1
-0.3
-0.3
-0.2
growth on oleic acid
YNB Glucose
+0.5
-0.4
+0.0
-0.2
-0.3
growth on ricinoleic acid
YNB Glucose
+0.1
-0.4
-0.0
+0.1
-0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.0
-0.2
-0.0
+0.2
-0.1
ypd buoyancy
40 hrs High Buoyancy
-0.3
+0.0
+0.0
-0.1
+0.3
growth on ypd
YPD
-0.5
-0.2
+0.0
+0.9
-0.2
facs on lipid accumulation
Sorted No BODIPY Gate
+0.8
-0.1
-0.4
-0.3
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
+0.3
+0.3
+0.4
+0.4
-1.3
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.4
-0.5
-0.3
+0.6
+0.0
auxotrophy
YNB Glucose
+0.5
+0.1
-0.2
-0.0
+0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.6
+0.3
+0.4
+0.2
-0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.8
+0.2
+0.1
+0.1
+0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+1.1
+0.5
+0.2
-0.3
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.9
+0.5
+0.1
-0.0
+0.1
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