Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10896 and 10897 are separated by 159 nucleotides10897 and 10898 are separated by 272 nucleotides10898 and 10899 are separated by 1622 nucleotides10899 and 10900 are separated by 173 nucleotides mRNA_2528: 10896 - HMMPfam-Fungal chitosanase of glycosyl hydrolase group 75-PF07335, at 365,082 to 366,780 10896 mRNA_2529: 10897 - K01613 psd, PISD phosphatidylserine decarboxylase, at 366,940 to 368,677 10897 mRNA_2530: 10898 - K14634 TIGAR fructose-2,6-bisphosphatase, at 368,950 to 370,646 10898 mRNA_2531: 10899 - KOG2816 Predicted transporter ADD1 (major facilitator superfamily), at 372,269 to 375,397 10899 mRNA_2532: 10900 - KOG2961 Predicted hydrolase (HAD superfamily), at 375,571 to 377,208 10900
Group Condition mRNA_2528 mRNA_2529 mRNA_2530 mRNA_2531 mRNA_2532
10896 10897 10898 10899 10900
auxotrophy Dropout Complete +0.1 -0.5 -0.4 -0.2 -0.6
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 -0.2 -0.1 -1.1
growth on oleic acid YNB Oleic Acid +0.0 -0.3 -0.3 -0.2 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.5 -0.1 +0.2 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.4 -0.2 +0.6 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.2 +0.1 +0.1 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.1 +0.3 -0.4 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.1 -0.3 -0.3 -0.2
growth on oleic acid YNB Glucose +0.5 -0.4 +0.0 -0.2 -0.3
growth on ricinoleic acid YNB Glucose +0.1 -0.4 -0.0 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.2 -0.0 +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.0 +0.0 -0.1 +0.3
growth on ypd YPD -0.5 -0.2 +0.0 +0.9 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.8 -0.1 -0.4 -0.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.3 +0.4 +0.4 -1.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.5 -0.3 +0.6 +0.0
auxotrophy YNB Glucose +0.5 +0.1 -0.2 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 +0.3 +0.4 +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.8 +0.2 +0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 +0.5 +0.2 -0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.9 +0.5 +0.1 -0.0 +0.1
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