Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10900 and 10901 are separated by 247 nucleotides10901 and 10902 are separated by 381 nucleotides10902 and 10903 are separated by 22 nucleotides10903 and 10904 are separated by 359 nucleotides mRNA_2532: 10900 - KOG2961 Predicted hydrolase (HAD superfamily), at 375,571 to 377,208 10900 mRNA_2533: 10901 - K10706 SETX, ALS4 senataxin, at 377,456 to 384,141 10901 mRNA_2534: 10902 - HMMPfam-Nucleotide-diphospho-sugar transferase-PF03407, at 384,523 to 387,549 10902 mRNA_2535: 10903 - Hypothetical Protein, at 387,572 to 388,066 10903 mRNA_2536: 10904 - K15601 KDM3 lysine-specific demethylase 3, at 388,426 to 391,874 10904
Group Condition mRNA_2532 mRNA_2533 mRNA_2534 mRNA_2535 mRNA_2536
10900 10901 10902 10903 10904
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.4 -0.1 -0.1 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.5 +0.2 +0.1 -1.2
auxotrophy Dropout Complete -0.6 -0.1 -0.4 -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine -1.1 -0.2 +0.2 +0.2 -0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.3 -0.2 -0.1 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 -0.0 +0.1 -1.0
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 +0.0 +0.0 -0.2 +0.6
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 -0.1 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.3 -0.1 -0.2 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.0 -0.1 -0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.4 +0.1 +0.2 -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.1 +0.0 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.0 +0.1 -0.1 +0.3
auxotrophy YNB Glucose +0.2 -0.4 +0.0 +0.2 -0.1
growth on oleic acid YNB Glucose -0.3 -0.2 +0.2 -0.0 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 -0.1 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.8 +0.4 +0.3 -0.2 +0.2
growth on ypd YPD -0.2 +0.0 +0.1 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.4 +0.3 -0.0 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 +0.2 +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.0 +0.0 +0.0 +0.2
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