Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10913 and 10914 are separated by 550 nucleotides10914 and 10915 are separated by 249 nucleotides10915 and 10916 are separated by 206 nucleotides10916 and 10917 are separated by 110 nucleotides mRNA_2545: 10913 - K05767 IQGAP2_3 Ras GTPase-activating-like protein IQGAP2/3, at 407,193 to 413,083 10913 mRNA_2546: 10914 - Hypothetical Protein, at 413,634 to 416,302 10914 mRNA_2547: 10915 - HMMPfam-Protein kinase domain-PF00069,ProSiteProfiles-Protein kinase domain profile.-PS50011,SUPERFAMILY--SSF56112, at 416,552 to 418,992 10915 mRNA_2548: 10916 - KOG1943 Beta-tubulin folding cofactor D, at 419,199 to 423,600 10916 mRNA_2549: 10917 - HMMPfam-Chromatin modification-related protein EAF7-PF07904, at 423,711 to 424,964 10917
Group Condition mRNA_2545 mRNA_2546 mRNA_2547 mRNA_2548 mRNA_2549
10913 10914 10915 10916 10917
auxotrophy Dropout Complete -1.5 -0.3 -0.7 -0.1 -0.8
auxotrophy YNB Glucose -2.0 +0.0 -0.2 +0.2 -0.7
facs on lipid accumulation Sorted No BODIPY Gate -1.9 +0.2 +0.2 +0.2 -1.4
facs on lipid accumulation Sorted High BODIPY Gate -0.9 +0.1 -0.1 -0.8 -0.9
auxotrophy YNB Glucose 75mM Methionine -1.8 +0.0 -0.3 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -1.3 -0.3 -0.2 -0.1 -0.4
growth on oleic acid YNB Glucose -1.0 +0.1 -0.1 -0.4 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -1.7 -0.4 -0.5 +1.6 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.6 -0.1 +0.4 -0.2 +0.7
growth on oleic acid YNB Oleic Acid -0.2 +0.0 -0.3 +0.5 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.4 -0.1 +0.1 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.0 +0.5 -0.1 -0.6 +1.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 +0.0 -0.2 +0.7 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.1 +0.2 -1.2 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 +0.5 +0.1 -0.4 +0.6
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.0 +0.1 +0.5 -0.2
growth on ricinoleic acid YNB Glucose -0.4 -0.1 -0.1 +0.2 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.0 +0.1 +0.8 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.1 -0.1 +0.6 +1.2
growth on ypd YPD +1.1 +0.0 -0.0 -0.8 +1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.4 +0.0 -0.8 +2.2
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