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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10914 and 10915 are separated by 249 nucleotides
10915 and 10916 are separated by 206 nucleotides
10916 and 10917 are separated by 110 nucleotides
10917 and 10918 are separated by 210 nucleotides
mRNA_2546: 10914 - Hypothetical Protein, at 413,634 to 416,302
10914
mRNA_2547: 10915 - HMMPfam-Protein kinase domain-PF00069,ProSiteProfiles-Protein kinase domain profile.-PS50011,SUPERFAMILY--SSF56112, at 416,552 to 418,992
10915
mRNA_2548: 10916 - KOG1943 Beta-tubulin folding cofactor D, at 419,199 to 423,600
10916
mRNA_2549: 10917 - HMMPfam-Chromatin modification-related protein EAF7-PF07904, at 423,711 to 424,964
10917
mRNA_2550: 10918 - KOG3116 Predicted C3H1-type Zn-finger protein, at 425,175 to 426,192
10918
Group
Condition
mRNA
_2546
mRNA
_2547
mRNA
_2548
mRNA
_2549
mRNA
_2550
10914
10915
10916
10917
10918
auxotrophy
Dropout Complete
-0.3
-0.7
-0.1
-0.8
-0.4
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
-0.1
-0.8
-0.9
-0.3
auxotrophy
YNB Glucose 75mM Arginine
-0.3
-0.2
-0.1
-0.4
-0.5
ypd buoyancy
40 hrs No Buoyancy Separation
-0.4
-0.5
+1.6
+0.1
-2.3
facs on lipid accumulation
Sorted No BODIPY Gate
+0.2
+0.2
+0.2
-1.4
-0.4
auxotrophy
YNB Glucose
+0.0
-0.2
+0.2
-0.7
-0.1
growth on oleic acid
YNB Oleic Acid
+0.0
-0.3
+0.5
-0.4
-0.5
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
+0.2
-1.2
+0.7
-0.4
auxotrophy
YNB Glucose 75mM Methionine
+0.0
-0.3
-0.1
-0.1
+0.0
growth on oleic acid
YNB Glucose
+0.1
-0.1
-0.4
-0.0
+0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.1
+0.1
-0.0
-0.1
growth on ypd
YPD
+0.0
-0.0
-0.8
+1.0
-0.0
growth on ricinoleic acid
YNB Glucose
-0.1
-0.1
+0.2
+0.5
-0.2
ypd buoyancy
40 hrs High Buoyancy
+0.0
+0.1
+0.8
-0.2
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
+0.4
-0.2
+0.7
-0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.0
+0.1
+0.5
-0.2
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.5
-0.1
-0.6
+1.0
+0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
+0.0
-0.8
+2.2
-1.0
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.0
-0.2
+0.7
+0.4
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.5
+0.1
-0.4
+0.6
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-0.1
+0.6
+1.2
-0.1
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