Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10914 and 10915 are separated by 249 nucleotides10915 and 10916 are separated by 206 nucleotides10916 and 10917 are separated by 110 nucleotides10917 and 10918 are separated by 210 nucleotides mRNA_2546: 10914 - Hypothetical Protein, at 413,634 to 416,302 10914 mRNA_2547: 10915 - HMMPfam-Protein kinase domain-PF00069,ProSiteProfiles-Protein kinase domain profile.-PS50011,SUPERFAMILY--SSF56112, at 416,552 to 418,992 10915 mRNA_2548: 10916 - KOG1943 Beta-tubulin folding cofactor D, at 419,199 to 423,600 10916 mRNA_2549: 10917 - HMMPfam-Chromatin modification-related protein EAF7-PF07904, at 423,711 to 424,964 10917 mRNA_2550: 10918 - KOG3116 Predicted C3H1-type Zn-finger protein, at 425,175 to 426,192 10918
Group Condition mRNA_2546 mRNA_2547 mRNA_2548 mRNA_2549 mRNA_2550
10914 10915 10916 10917 10918
auxotrophy Dropout Complete -0.3 -0.7 -0.1 -0.8 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.1 -0.8 -0.9 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.2 -0.1 -0.4 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.5 +1.6 +0.1 -2.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.2 +0.2 -1.4 -0.4
auxotrophy YNB Glucose +0.0 -0.2 +0.2 -0.7 -0.1
growth on oleic acid YNB Oleic Acid +0.0 -0.3 +0.5 -0.4 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.2 -1.2 +0.7 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.3 -0.1 -0.1 +0.0
growth on oleic acid YNB Glucose +0.1 -0.1 -0.4 -0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 +0.1 -0.0 -0.1
growth on ypd YPD +0.0 -0.0 -0.8 +1.0 -0.0
growth on ricinoleic acid YNB Glucose -0.1 -0.1 +0.2 +0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 +0.8 -0.2 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.4 -0.2 +0.7 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 +0.5 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.1 -0.6 +1.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.0 -0.8 +2.2 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.2 +0.7 +0.4 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.1 -0.4 +0.6 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 +0.6 +1.2 -0.1
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