Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10939 and 10940 overlap by 32 nucleotides10940 and 10941 are separated by 196 nucleotides10941 and 10942 are separated by 130 nucleotides10942 and 10943 are separated by 131 nucleotides mRNA_2571: 10939 - HMMPfam-Methyltransferase domain-PF13679, at 470,466 to 472,507 10939 mRNA_2572: 10940 - K13093 HTATSF1 HIV Tat-specific factor 1, at 472,476 to 474,035 10940 mRNA_2573: 10941 - K03848 ALG6 alpha-1,3-glucosyltransferase, at 474,232 to 477,732 10941 mRNA_2574: 10942 - K10158 B3GAT3 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, at 477,863 to 478,879 10942 mRNA_2575: 10943 - KOG1022 Acetylglucosaminyltransferase EXT2/exostosin 2, at 479,011 to 480,812 10943
Group Condition mRNA_2571 mRNA_2572 mRNA_2573 mRNA_2574 mRNA_2575
10939 10940 10941 10942 10943
ypd buoyancy 40 hrs No Buoyancy Separation -1.7 N.D. -0.2 -1.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.2 -1.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.0 -0.9 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.1 -0.5 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.1 -0.2 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 -0.4 -0.2
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.0 -0.3 +0.0
auxotrophy Dropout Complete +0.1 N.D. -0.1 -0.5 +0.1
growth on ypd YPD +0.4 N.D. -0.1 -0.2 -0.2
auxotrophy YNB Glucose +0.0 N.D. -0.0 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. -0.1 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. -0.1 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.1 -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.1 -0.0 +0.1
growth on oleic acid YNB Glucose -0.1 N.D. +0.0 +0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.1 +0.2 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.0 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. -0.2 +0.4 +0.2
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.0 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. -0.1 +0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.1 +0.4 +0.2
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