Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10941 and 10942 are separated by 130 nucleotides10942 and 10943 are separated by 131 nucleotides10943 and 10944 are separated by 72 nucleotides10944 and 10945 are separated by 153 nucleotides mRNA_2573: 10941 - K03848 ALG6 alpha-1,3-glucosyltransferase, at 474,232 to 477,732 10941 mRNA_2574: 10942 - K10158 B3GAT3 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, at 477,863 to 478,879 10942 mRNA_2575: 10943 - KOG1022 Acetylglucosaminyltransferase EXT2/exostosin 2, at 479,011 to 480,812 10943 mRNA_2576: 10944 - Hypothetical Protein, at 480,885 to 482,213 10944 mRNA_2577: 10945 - HMMPfam-Protein of unknown function (DUF3767)-PF12597, at 482,367 to 483,175 10945
Group Condition mRNA_2573 mRNA_2574 mRNA_2575 mRNA_2576 mRNA_2577
10941 10942 10943 10944 10945
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 -0.0 +0.1 -1.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -1.2 -0.3 +0.0 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.9 -0.7 -0.0 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -1.1 +0.2 -0.5 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.4 +0.2 +0.4 -1.6
growth on oleic acid YNB Oleic Acid -0.0 -0.3 +0.0 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.4 -0.2 -0.3 +0.3
auxotrophy Dropout Complete -0.1 -0.5 +0.1 -0.2 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.5 -0.4 -0.3 +0.8
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 -0.2 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 -0.2 -0.2 +0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.1 +0.2 +0.2 -0.4
growth on ypd YPD -0.1 -0.2 -0.2 +0.0 +0.7
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 +0.1 +0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.1 -0.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.4 -0.1 +1.1
growth on oleic acid YNB Glucose +0.0 +0.2 +0.1 -0.3 +0.4
auxotrophy YNB Glucose -0.0 -0.1 -0.1 +0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.0 -0.0 +0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 +0.2 +0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.4 +0.2 +0.2 +0.3
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