Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10943 and 10944 are separated by 72 nucleotides10944 and 10945 are separated by 153 nucleotides10945 and 10946 are separated by 460 nucleotides10946 and 10947 are separated by 134 nucleotides mRNA_2575: 10943 - KOG1022 Acetylglucosaminyltransferase EXT2/exostosin 2, at 479,011 to 480,812 10943 mRNA_2576: 10944 - Hypothetical Protein, at 480,885 to 482,213 10944 mRNA_2577: 10945 - HMMPfam-Protein of unknown function (DUF3767)-PF12597, at 482,367 to 483,175 10945 mRNA_2578: 10946 - KOG3574 Acetyl-CoA transporter, at 483,636 to 486,060 10946 mRNA_2579: 10947 - K10597 UBE4B, UFD2 ubiquitin conjugation factor E4 B, at 486,195 to 490,612 10947
Group Condition mRNA_2575 mRNA_2576 mRNA_2577 mRNA_2578 mRNA_2579
10943 10944 10945 10946 10947
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.1 -1.5 +0.9 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.0 +0.4 -0.3 -1.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.4 -1.6 +1.0 -1.2
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.0 -0.1 +0.0 -0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.7 -0.0 +0.5 +0.0 -0.8
auxotrophy Dropout Complete +0.1 -0.2 +0.4 -0.0 -1.1
growth on oleic acid YNB Oleic Acid +0.0 -0.2 -0.2 +0.5 -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.3 +0.8 -0.2 -0.5
growth on ricinoleic acid YNB Glucose +0.2 +0.2 -0.4 +0.7 -1.2
growth on oleic acid YNB Glucose +0.1 -0.3 +0.4 +0.3 -0.9
auxotrophy YNB Glucose -0.1 +0.3 +0.3 +0.4 -1.3
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.1 +0.4 +0.4 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.3 +0.3 +0.0 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.5 +0.6 -0.6 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.1 +1.1 +0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.2 +0.1 +0.4 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 -0.0 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 -0.0 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.2 +0.3 +0.3 -0.3
growth on ypd YPD -0.2 +0.0 +0.7 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.2 +0.1 +0.3 +0.6
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