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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10943 and 10944 are separated by 72 nucleotides
10944 and 10945 are separated by 153 nucleotides
10945 and 10946 are separated by 460 nucleotides
10946 and 10947 are separated by 134 nucleotides
mRNA_2575: 10943 - KOG1022 Acetylglucosaminyltransferase EXT2/exostosin 2, at 479,011 to 480,812
10943
mRNA_2576: 10944 - Hypothetical Protein, at 480,885 to 482,213
10944
mRNA_2577: 10945 - HMMPfam-Protein of unknown function (DUF3767)-PF12597, at 482,367 to 483,175
10945
mRNA_2578: 10946 - KOG3574 Acetyl-CoA transporter, at 483,636 to 486,060
10946
mRNA_2579: 10947 - K10597 UBE4B, UFD2 ubiquitin conjugation factor E4 B, at 486,195 to 490,612
10947
Group
Condition
mRNA
_2575
mRNA
_2576
mRNA
_2577
mRNA
_2578
mRNA
_2579
10943
10944
10945
10946
10947
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.0
+0.1
-1.5
+0.9
-1.0
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.3
+0.0
+0.4
-0.3
-1.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
+0.4
-1.6
+1.0
-1.2
auxotrophy
YNB Glucose 75mM Arginine
-0.2
-0.0
-0.1
+0.0
-0.9
facs on lipid accumulation
Sorted No BODIPY Gate
-0.7
-0.0
+0.5
+0.0
-0.8
auxotrophy
Dropout Complete
+0.1
-0.2
+0.4
-0.0
-1.1
growth on oleic acid
YNB Oleic Acid
+0.0
-0.2
-0.2
+0.5
-0.9
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
-0.3
+0.8
-0.2
-0.5
growth on ricinoleic acid
YNB Glucose
+0.2
+0.2
-0.4
+0.7
-1.2
growth on oleic acid
YNB Glucose
+0.1
-0.3
+0.4
+0.3
-0.9
auxotrophy
YNB Glucose
-0.1
+0.3
+0.3
+0.4
-1.3
auxotrophy
YNB Glucose 75mM Methionine
-0.0
+0.1
+0.4
+0.4
-1.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.3
+0.3
+0.0
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
+0.2
-0.5
+0.6
-0.6
+0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.4
-0.1
+1.1
+0.2
-0.4
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
+0.2
+0.1
+0.4
-0.1
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.1
-0.0
+0.1
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.2
+0.1
-0.0
+0.1
+0.1
ypd buoyancy
40 hrs Low Buoyancy
+0.2
+0.2
+0.3
+0.3
-0.3
growth on ypd
YPD
-0.2
+0.0
+0.7
+0.3
-0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
-0.2
+0.1
+0.3
+0.6
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