Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10949 and 10950 are separated by 48 nucleotides10950 and 10951 are separated by 102 nucleotides10951 and 10952 are separated by 239 nucleotides10952 and 10953 are separated by 738 nucleotides mRNA_2581: 10949 - K06096 VAPA vesicle-associated membrane protein-associated protein A, at 492,942 to 494,422 10949 mRNA_2582: 10950 - HMMPfam-Ribosomal protein L35-PF01632,PRINTS-Ribosomal protein L35 signature-PR00064,ProSitePatterns-Ribosomal protein L35 signature.-PS00936,SUPERFAMILY--SSF143034, at 494,471 to 495,097 10950 mRNA_2583: 10951 - K10610 DDB1 DNA damage-binding protein 1, at 495,200 to 499,404 10951 mRNA_2584: 10952 - K12350 SMPD1, ASM sphingomyelin phosphodiesterase, at 499,644 to 502,553 10952 mRNA_2585: 10953 - K18167 SDHAF1 succinate dehydrogenase assembly factor 1, at 503,292 to 503,677 10953
Group Condition mRNA_2581 mRNA_2582 mRNA_2583 mRNA_2584 mRNA_2585
10949 10950 10951 10952 10953
growth on oleic acid YNB Oleic Acid -0.1 -0.6 -0.4 +0.2 -0.9
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.2 -0.3 +0.3 -1.1
auxotrophy Dropout Complete -0.5 +0.0 -0.6 +0.2 -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.3 -0.4 -0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 +0.2 +0.2 +0.2 -1.1
growth on oleic acid YNB Glucose -0.4 -0.1 -0.2 +0.2 -0.3
growth on ricinoleic acid YNB Glucose -0.3 -0.2 -0.3 +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.6 -0.6 +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.7 +0.1 -0.3 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -1.1 -0.8 +0.3 +1.1
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -0.0 -0.4 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 -0.1 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.6 -0.3 -0.1 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.4 -0.4 -0.1 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 -0.3 -0.0 +0.2
auxotrophy YNB Glucose -0.3 +0.5 -0.3 +0.3 -0.1
growth on ypd YPD -0.3 -0.1 -0.0 +0.2 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.2 +0.0 +0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.4 +0.1 -0.0 -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 +0.0 -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.3 -0.2 +0.4 +0.4
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