Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10952 and 10953 are separated by 738 nucleotides10953 and 10954 are separated by 93 nucleotides10954 and 10955 are separated by 38287 nucleotides10955 and 10956 are separated by 291 nucleotides mRNA_2584: 10952 - K12350 SMPD1, ASM sphingomyelin phosphodiesterase, at 499,644 to 502,553 10952 mRNA_2585: 10953 - K18167 SDHAF1 succinate dehydrogenase assembly factor 1, at 503,292 to 503,677 10953 mRNA_2586: 10954 - K05765 CFL cofilin, at 503,771 to 504,788 10954 mRNA_2587: 10955 - K04368 MAP2K1, MEK1 mitogen-activated protein kinase kinase 1, at 543,076 to 544,917 10955 mRNA_2588: 10956 - K03246 EIF3I translation initiation factor 3 subunit I, at 545,209 to 547,043 10956
Group Condition mRNA_2584 mRNA_2585 mRNA_2586 mRNA_2587 mRNA_2588
10952 10953 10954 10955 10956
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.2 N.D. -4.8 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.2 N.D. -3.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +1.1 N.D. -5.0 N.D.
auxotrophy YNB Glucose +0.3 -0.1 N.D. -3.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -1.1 N.D. -2.4 N.D.
growth on oleic acid YNB Glucose +0.2 -0.3 N.D. -3.0 N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.9 N.D. -2.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 N.D. -2.8 N.D.
auxotrophy Dropout Complete +0.2 -0.7 N.D. -1.9 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.2 N.D. -2.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.4 N.D. -2.9 N.D.
growth on ypd YPD +0.2 +0.6 N.D. -2.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 N.D. -2.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.3 N.D. -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.5 N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.8 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -1.1 N.D. +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 N.D. -0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.3 N.D. +0.8 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.3 N.D. +1.7 N.D.
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