Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10954 and 10955 are separated by 38287 nucleotides10955 and 10956 are separated by 291 nucleotides10956 and 10957 are separated by 949 nucleotides10957 and 10958 are separated by 505 nucleotides mRNA_2586: 10954 - K05765 CFL cofilin, at 503,771 to 504,788 10954 mRNA_2587: 10955 - K04368 MAP2K1, MEK1 mitogen-activated protein kinase kinase 1, at 543,076 to 544,917 10955 mRNA_2588: 10956 - K03246 EIF3I translation initiation factor 3 subunit I, at 545,209 to 547,043 10956 mRNA_2589: 10957 - Hypothetical Protein, at 547,993 to 548,642 10957 mRNA_2590: 10958 - Hypothetical Protein, at 549,148 to 549,789 10958
Group Condition mRNA_2586 mRNA_2587 mRNA_2588 mRNA_2589 mRNA_2590
10954 10955 10956 10957 10958
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -4.8 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -5.0 N.D. +0.6 +0.0
growth on ricinoleic acid YNB Glucose N.D. -3.9 N.D. +0.2 +0.1
auxotrophy YNB Glucose N.D. -3.6 N.D. +0.7 +0.1
growth on oleic acid YNB Glucose N.D. -3.0 N.D. +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -2.8 N.D. +0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -2.4 N.D. +0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. -2.0 N.D. -0.2 -0.0
growth on ypd YPD N.D. -2.6 N.D. +0.6 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -2.9 N.D. +0.8 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -2.1 N.D. +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -2.0 N.D. +0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -1.0 N.D. -0.6 -0.0
auxotrophy Dropout Complete N.D. -1.9 N.D. +0.7 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.5 N.D. -0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 N.D. +0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 N.D. -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 N.D. +0.6 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.8 N.D. +0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.7 N.D. -0.3 +0.0
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