Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10973 and 10974 are separated by 682 nucleotides10974 and 10975 are separated by 289 nucleotides10975 and 10976 are separated by 1099 nucleotides10976 and 10977 are separated by 2979 nucleotides mRNA_2605: 10973 - K02370 EXTL3 alpha-1,4-N-acetylglucosaminyltransferase EXTL3, at 577,060 to 580,725 10973 mRNA_2606: 10974 - Hypothetical Protein, at 581,408 to 582,467 10974 mRNA_2607: 10975 - KOG4102 Uncharacterized conserved protein, at 582,757 to 583,405 10975 mRNA_2608: 10976 - Hypothetical Protein, at 584,505 to 585,992 10976 mRNA_2609: 10977 - Hypothetical Protein, at 588,972 to 589,930 10977
Group Condition mRNA_2605 mRNA_2606 mRNA_2607 mRNA_2608 mRNA_2609
10973 10974 10975 10976 10977
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.3 N.D. -1.1 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.5 N.D. +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.3 N.D. +0.1 -0.6
auxotrophy Dropout Complete +0.0 -0.0 N.D. -0.5 +0.1
growth on oleic acid YNB Glucose +0.1 -0.2 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 N.D. -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 -0.0 N.D. +0.4 +0.2
growth on ypd YPD -0.0 -0.3 N.D. +0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.0 N.D. +0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.0 N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.0 N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.3 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. -0.0 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.2 N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.2 N.D. +0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.0 N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.4 N.D. +0.0 +0.1
growth on ricinoleic acid YNB Glucose +0.3 +0.1 N.D. -0.0 +0.2
auxotrophy YNB Glucose +0.3 +0.0 N.D. -0.2 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.0 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.3 N.D. +0.2 +0.3
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