Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10980 and 10981 are separated by 239 nucleotides10981 and 10982 are separated by 571 nucleotides10982 and 10983 are separated by 935 nucleotides10983 and 10984 are separated by 448 nucleotides mRNA_2612: 10980 - HMMPfam-Protein kinase C terminal domain-PF00433, at 591,842 to 594,740 10980 mRNA_2613: 10981 - K00223 ERG4 Delta24(24(1))-sterol reductase, at 594,980 to 596,945 10981 mRNA_2614: 10982 - K18050 PRKCE novel protein kinase C epsilon type, at 597,517 to 601,558 10982 mRNA_2615: 10983 - K14775 UTP30, RSL1D1 ribosome biogenesis protein UTP30, at 602,494 to 603,879 10983 mRNA_2616: 10984 - KOG4076 Regulator of ATP-sensitive K+ channels Alpha-endosulfine/ARPP-19 and related cAMP-regulated phosphoproteins, at 604,328 to 604,979 10984
Group Condition mRNA_2612 mRNA_2613 mRNA_2614 mRNA_2615 mRNA_2616
10980 10981 10982 10983 10984
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.4 N.D. -4.0
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.2 N.D. -4.3
auxotrophy Dropout Complete -0.1 N.D. -3.2 N.D. -0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -1.0 N.D. -2.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.4 N.D. -3.3
auxotrophy YNB Glucose +0.1 N.D. -3.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -3.2 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -3.1 N.D. -0.4
growth on oleic acid YNB Glucose -0.2 N.D. -2.8 N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.9 N.D. -0.5
growth on oleic acid YNB Oleic Acid -0.1 N.D. -2.2 N.D. +0.9
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -1.0 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. -1.1 N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -1.0 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.7 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. +0.0 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.5 N.D. +0.2
growth on ypd YPD -0.1 N.D. +0.2 N.D. -0.5
growth on ricinoleic acid YNB Glucose -0.3 N.D. -1.0 N.D. +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. +0.9 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.6 N.D. +0.1
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